Pioneer Hi-bred International, Inc.

Weining Gu

Zuo-Yu Zhao

Dorothy A Pierce

Tishu Cai

The present invention relates to methods for improving the transformation frequency of -mediated transformation of maize embryos. A preferred method for transforming maize using comprises the steps of: contacting at least one immature embryo from a maize plant with capable of transferring at least one gene to said embryo; co-cultivating the embryos with culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

Methods for Agrobacterium-mediated transformation

The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

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Urbandale, IA, United States of America

Weining Gu