Other

Chinese Academy of Agricultural Sciences

Beijing Tide Pharmaceutical Co., Ltd.

Weina Liu

Yuan Wang

Bin Yao

Huiying Luo

Kun Meng

Yaru Wang

Huoqing Huang

Yingguo Bai

Xiaoyun Su

Tao Tu

Jing Li

Jing Zhao

Hongjun Wang

Yanping Zhao

Liying Zhou

Yanan Liu

Yuanyuan Jiang

Weiting Zhong

Yuan Gu

Jianzhong Ge

The present invention falls within the field of biological medicine, and specifically relates to a method for preventing, alleviating and/or treating fatty liver disease and/or steatohepatitis. The method comprises administering, to an individual in need thereof, an effective amount of a compound of formula (I) or a pharmaceutically acceptable salt, an ester, a stereoisomer, a polymorph, a solvate, an N-oxide, an isotopically labeled compound, a metabolite or a prodrug thereof.

Method for treating fatty liver disease and/or steatohepatitis

The present invention relates to a mannanase PMan5A mutant having improved heat resistance, a gene encoding the mutant and application thereof. The mutant is obtained by a substitution of histidine with tyrosine at amino acid residue 93, phenylalanine with tyrosine at amino acid residue 94, leucine with histidine at amino acid residue 356, and/or alanine with proline at amino acid residue 389. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild-type mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play an important role for the thermal stability of the mannanase of GH5 family.

Mannanase PMAN5A mutant having improved heat resistance, gene thereof, and application

Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO:1 or SEQ ID NO:2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.

Glucose oxidase CnGODA and gene and application thereof

Growing community of inventors

Beijing, China

Weina Liu