Northwest University

Weina Li

Daidi Fan

Chenhui Zhu

A β-glycosidase SS-BGL mutant for modifying ginsenoside and application thereof are provided, which relate to the field of genetic engineering technologies. The β-glycosidase SS-BGL mutant is β-glycosidase SS-BGL with the amino acid sequence as shown in SEQ ID NO: 1 having mutation at one or more sites. A single-site mutation includes: A1, making glutamine at 96position of SEQ ID NO: 1 to be mutated to glutamic acid; A2, making asparagine at 97position of SEQ ID NO: 1 to be mutated to aspartate; A3, making asparagine at 128position of SEQ ID NO: 1 to be mutated to aspartate; A4, making asparagine at 302position of SEQ ID NO: 1 to be mutated to aspartate. A double-site mutation is a combination of A3 and A4, a three-site mutation is a combination of A1, A2 and A4, and a four-site mutation is a combination of A1, A2, A3 and A4.

Beta-glycosidase SS-BGL mutant for modifying ginsenoside and application thereof

A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125, 178, 313, 125and 178, or 125and 313positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.

Growing community of inventors

Xi'an, China

Weina Li