Mgi Tech Co., Ltd.

Bgi Shenzhen

Weimao Wang

Wei Chen

Jian Wang

Wenwei Zhang

Xun Xu

Mei Li

Ou Wang

Mengzhe Shen

Xiaofang Cheng

Qing Xie

Yuxiang Li

Fei Fan

Luman Cui

Wenwei Zhan

Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S).

Magnetic bead-based detection method, storage medium, and detection device

A method for obtaining a single-cell mRNA sequence, including: (1) capturing mRNA of a cell by using a cell tag carrier, and performing reverse transcription to obtain cDNA having a cell tag; (2) obtaining multiple cDNA fragments having molecular tags by using a transposase complex and a molecular tag carrier; (3) performing high-throughput sequencing; (4) performing sequence assembly according to the molecular tags to obtain the sequence of each mRNA; and () obtaining the sequence of all mRNAs of each single cell according to the cell tags.

Method for obtaining single-cell mRNA sequence

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Shenzhen, China

Weimao Wang