Technische Universitaet Dresden

Dynabind Gmbh

Technische Universitát Dresden

Weilin Lin

Yixin Zhang

Francesco Reddavide

Elisa Mele

Robert Wieduwild

Jana Herrmann

Helena De Andrade

Luca Mannocci

The present invention relates to a method for the sensitive identification of high-affinity complexes made of two ligands () and one receptor (). A large number of different ligands () of a chemical library are hereby contacted with at least one receptor () in a solution. The ligands of the library have a single-strand DNA () or RNA with a base length of 2 to 10 bases or alternatively more than 10 bases. In addition, the solution is incubated for a specific period of time and complexes made of two ligands () and one receptor () are identified.

Method for identifying high-affinity complexes made of two ligands and one receptor, device for implementing the method and self-assembling chemical library for use in the method

In a method and arrangement for detecting binding events of molecules, at least one second molecule covalently bound to a ligand, and optionally bonded to a third molecule bound to a ligand, forms a specific non-covalent fond with a first molecule immobilised on a bioactive surface by a covalent bond, and binding events between an analyte molecule and the second-molecule bound ligand are detected by an analytical measuring method, wherein the specific non-covalent bond between the first and second molecules is breakable by supplying a buffer solution, and, hence, a specific ligand, or two, different specific ligands are made available on the bioactive surface in an easily reversible manner.

Method and arrangement for detecting binding events of molecules

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Dresden, Germany

Weilin Lin