Institute of Animal Sciences of Caas

Weifeng Yuan

Hongfei Zhu

Zhaoyang Wang

Hong Jia

Qianqian Feng

Ting Xin

Xiaoyu Guo

Zhongbao Pang

Shaohua Hou

Yitong Jiang

A heavy-chain variable region sequence of the nanobody CPV-VHH-H1 has the amino acid sequence set forth in SEQ ID NO: 1; and a gene encoding the nanobody CPV-VHH-H1 has the nucleotide sequence set forth in SEQ ID NO: 2. A nanobody immune library of the CPV is constructed by a phage display technology, a specific anti-CPV nanobody CPV-VHH-H1 is obtained by screening, and it is verified by experiments that the nanobody may specifically bind to the CPV. A new nanobody preparation for use in clinical diagnosis and treatment of the CPV can be developed, and a certain theoretical reserve is provided for applying the nanobody to the field of veterinary biological products.

Canine parvovirus (CPV) nanobody CPV-VHH-H1 and use thereof

Provided are a canine parvovirus (CPV) nanobody CPV-VHH-E3 and application thereof, belonging to the technical field of immunology. The nanobody CPV-VHH-E3 includes heavy chain variable region with amino acid sequence as shown in SEQ ID NO: 1, and a nucleotide sequence of a gene encoding the nanobody CPV-VHH-E3 is shown in SEQ ID NO: 2. The present application constructs a nanobody immune library for CPV by phage-display technology, and obtains specific anti-CPV nanobody CPV-VHH-E3 by screening, which is verified to specifically bind CPV through experiments, and is applicable to develop a nanobody preparation for clinical diagnosis and treatment of CPV, providing a certain theoretical support for the application of nanobodies in the field of veterinary biological products.

Canine parvovirus nanobody CPV-VHH-E3 and application thereof

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Beijing, China

Weifeng Yuan