Canon U.S. Life Sciences, Inc.

Weidong Cao

Kenton C Hasson

Ivor T Knight

Takayoshi Hanagata

Hiroshi Inoue

Brian P Murphy

Eric D Schneider

Johnathan Stuart Coursey

Shulin Zeng

Alex K Flamm

Ben Lane

Gregory H Owen

Scott Corey

Sami Kanderian

Conrad Laskowski

Hongye Liang

Brian Bean

Franklin Regan

Ying-Xin Wang

Kevin Louder

Chris Felice

Ronald Kurz

Kazuhiko Hasegawa

Ralph McCann

Jason Clemons

The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

System and method for serial processing of multiple nucleic acid assays

The present invention relates to methods and systems for temperature correction in thermal melt analysis. More specifically, embodiments of the invention relate to the correction of the melting temperature (T) observed for a sample nucleic acid due to the effect of the nucleic acid concentration.

Method and system for temperature correction in thermal melt analysis

The present invention relates to methods and systems for microfluidic DNA sample preparation. More specifically, embodiments of the present invention relate to methods and systems for the isolation of DNA from patient samples on a microfluidic device and use of the DNA for downstream processing, such as performing amplification reactions and thermal melt analysis on the microfluidic device.

Methods and systems for microfluidic DNA sample preparation

Cartridge assembly

The present invention relates to a method for reducing cross-contamination in continuous amplification reactions in channels of microfluidic devices. More specifically, the present invention relates to the use of specific materials continuously flowing in the channels to reduce adsorption of MgCland the concomitant adsorption of nucleic acid template to the channel surface, thereby reducing cross-contamination. This reduction of cross-contamination improves the efficiency and reproducibility of the amplification reaction, e.g., PCR.

Method of reducing cross-contamination in continuous amplification reactions in a channel

The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for resolving a denaturation curve of a sample containing a first and second nucleic acid into a resolved denaturation curve for the first nucleic acid and a resolved denuration curve for the second nucleic acid.

High-resolution melting analysis

The present invention relates to novel unsymmetrical cyanine dyes and to methods of performing nucleic acid analysis in the presence of such dyes. More specifically, the present invention relates to novel unsymmetrical cyanine dyes that have high affinity to double-stranded nucleic acids and that do not inhibit amplification reactions, particularly the polymerase chain reaction (PCR).

Unsymmetrical cyanine dyes for high resolution nucleic acid melting analysis

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Rockville, MD, United States of America

Weidong Cao