Micromass UK Limited

Waters Technologies Corporation

Weibin Chen

John Charles Gebler

Jeffery Mark Brown

Asish B Chakraborty

Edouard S P Bouvier

James Ian Langridge

Jeff Brown

Emmanuelle Claude

Peter Jeng Jong Lee

Dominic Gostick

Scott Geromanos

Steven J Ciavarini

Jeffrey Mark Brown

Stephane Houel

Steven Cubbedge

Liuxi Chen

Edouard SP Bouvier

The present disclosure relates to a streamlined, complete workflow for the qualitative and the quantitative analysis of conjugated peptides from antibody-drug conjugate (ADC) compounds.

Identification and quantification of conjugated peptides in antibody drug conjugates by mass spectrometry

A mass spectrometer is disclosed wherein highly charged fragment ions resulting from Electron Transfer Dissociation fragmentation of parent ions are reduced in charge state within a Proton Transfer Reaction cell by reacting the fragment ions with a neutral superbase reagent gas such as Octahydropyrimidolazepine.

Method of charge reduction of electron transfer dissociation product ions

A method of mass spectrometry comprises ionizing a sample eluting from a separation device in order to generate a plurality of parent ions. The method further comprises generating a target list of ions, which includes a predicted mass to charge ratio, a predicted chromatographic retention or elution time, and a predicted ion mobility drift time, derived from a model. Multiple cycles of operation are then performed as the sample elutes from the separation device. Each cycle of operation includes mass filtering the parent ions so that selected ions having mass to charge ratios within a first mass to charge ratio range are onwardly transmitted to a fragmentation or reaction device. The target list is then checked and the model is updated accordingly. The first mass to charge ratio range can then be adjusted in response to the updated model.

Intelligent target-based acquisition

A mass spectrometer is disclosed wherein highly charged fragment ions resulting from Electron Transfer Dissociation fragmentation of parent ions are reduced in charge state within a Proton Transfer Reaction cellby reacting the fragment ions with a neutral superbase reagent gas such as Octahydropyrimidolazepine.

A MALDI sample plateis disclosed which comprises a metallic substratehaving a circular groove or moat. A hydrophobic polytetrafluoroethylene layeris applied to the substrateand a central portionof the substrateis laser etched which roughens the surface of the substrate. A thin polystyrene layer is then applied to the polytetrafluoroethylene layerand the central portion

MALDI sample plate

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Holliston, MA, United States of America

Weibin Chen