Advanced Analytical Technologies, Inc.

Agilent Technologies, Inc.

Iowa State University Research Foundation, Inc.

Conocophillips Company

Combisep, Inc.

Wei Wei

Ho-ming Pang

Bruce R Boeke

Edward S Yeung

Jay Edward Locklear

Martin Chris Foster

Mark D McClure

Thomas D Baugh

Pierre Varineau

Jolita J Uthe

Pang Ho-ming

Gary W Procop

Mark R Ver Meer

The invention is a multiplex, pulsed-field capillary electrophoresis instrument with the ability to analyze DNA fragments with sizes greater than 150,000 base pairs. The parallel capillary electrophoresis system allows for the simultaneous analysis of at least 12 samples while applying a pulse or varying electric field for separation. Sequences of pulse-field electric fields are iterated to achieve accurate separation of DNA smears.

Pulse-field multiplex capillary electrophoresis system

A multiple capillary florescent detection system employing optical fiber bundles that each fiber bundle has more than one fiber illuminating each sample vessel.

Bundled fiber optic capillary electrophoresis detection system

Pixelated 2-dimensional fluorescence detection system

A method for determining a concentration of a scale inhibitor in a water system that includes contacting a scale inhibitor with a dye in a capillary channel to form a complex effective to generate fluorescence; measuring a fluorescence signal generated by the complex; and, determining the concentration of the scale inhibitor based on the fluorescence signal.

Measurement of scale inhibitor in water systems

The invention is a high sensitivity and high throughput capillary multiwavelength fluorescence detection system. The fluorescent detection system is configured to illuminate a relatively large volume of a single capillary or a plurality of capillaries, with a pixelated detection system capable of imaging an area of each capillary that differentiates the capillary walls, the space between the capillaries, and the internal liquid volume within the capillary. Only the desired pixels or image area are used for processing and generating an output signal.

Capillary electrophoresis fluorescent detection system

The invention is a high-throughput multi-capillary system utilizing a 2-dimensional mask onto which the detection windows of the capillaries are lined up in alternating columns and rows. The image of the capillaries is projected onto a 2-dimensional detection system, such as a charge-coupled-detector (CCD) array. Each row and column of the capillary detection windows is imaged in a separate area of the CCD array, which results in reduced cross-talk between capillaries.

Method for the reduction of cross talk in multiplex capillary electrophoresis

Using multiple dichroics mirrors to split and redirect different wavelength regions of light in a multi-wavelength fluorescence detection system.

Multi-wavelength fluorescence detection system for multiplexed capillary electrophoresis

The invention includes a method of sample profiling for identification. The method includes the steps of performing less than four nucleotide-specific chemical cleavage reactions to obtain nucleotide sequence fragments, performing size separation on the fragments, detecting the fragments' separation, generating a profile based on the detection, and comparing the profile to a data base to identify the sample.

System and method for nucleotide sequence profiling for sample identification

A separation matrix for capillary electrophoresis of single-stranded nucleic acids is disclosed. The matrix comprises an aqueous buffer containing a denaturant and (hydroxypropyl)methyl cellulose.

Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations

A sieving medium for use in the separation of analytes in a sample containing at least one such analyte comprises a monomeric non-ionic surfactant of the of the general formula, B-A, wherein A is a hydrophilic moiety and B is a hydrophobic moiety, present in a solvent at a concentration forming a self-assembled micelle configuration under selected conditions and having an aggregation number providing an equivalent weight capable of effecting the size separation of the sample solution so as to resolve a target analyte(s) in a solution containing the same, the size separation taking place in a chromatography or electrophoresis separation system.

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Ames, IA, United States of America

Wei Wei