Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Allegro 3d, Inc.

Netflix Inc.

Wei Wei

Alexandre Richez

John M Beierle

Anthony Neal Park

Sean M Ramirez

Ludovic Vincent

Peyton Shieh

Edwin Li

Naiqian Zhan

Neil D Hunt

David Louis Heiner

James M Tsay

Sang Ryul Park

Kevan Samiee

Scott William Bailey

Tyler Jamison Dill

Anmiv Prabhu

Robert Yang

Yuxiang Huang

Kristina Munoz

Leonid Malevanchik

Samantha Schmitt

Sunmin Ahn

Valerie Uzzell

Hai Quang Tran

Randall Smith

Lewis J Kraft

Brian Douglas Mather

Xavier Von Hatten

Michael S Graige

Hong Xie

Gianluca Andrea Artioli

Julia Morrison

Wei Zhu

Maxwell Zimmerley

Hayden Black

Krystal Sly

Innsu Daniel Kim

Alexander Fuhrmann

Sojeong Moon

Ryan Sanford

Anthony N Park

Disclosed are methods and apparatuses for fabrication of polymer scaffolds and biological tissues in the multi-well plates in a rapid, high-throughput, controllable and reproducible manner by using optical exposure of the wells to patterned probe light without using a photomask. In some aspects, an apparatus includes a light source to produce a probe light; a digital display device to spatially modulate the probe light to encode a programmable spatial pattern in a spatially-modulated light; a stage to hold a target surface or chamber, wherein the target surface or chamber contains a solution including a material that forms a scaffold or construct based on interaction with the spatially-modulated light projected at the solution; and a computer control device in communication with the light source and the digital display device to control a change of the solution including the material to form the scaffold or construct.

Method and apparatus for high-throughput maskless fabrication of polymer scaffolds and biological tissues in multi-well plates

An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

Arrays with quality control tracers

A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

Flow cell package and method for making the same

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

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San Diego, CA, United States of America

Wei Wei