General Electric Company

Global Life Sciences Solutions Operations UK Ltd

Ge Healthcare Bio-sciences Corp.

Ge Healthcare UK Limited

Wei Gao

John Richard Nelson

Erik Leeming Kvam

Christopher Michael Puleo

Alex David Corwin

Todd Frederick Miller

David Andrew Shoudy

Kwok Pong Chan

Arunkumar Natarajan

Michael John Gerdes

Robert Scott Duthie

Christine Lynne Pitner

Ralf Lenigk

Brian Michael Davis

Ryan Charles Heller

Lakshmi Sireesha Kaanumalle

Abraham Joy

Nichole Lea Wood

Patrick McCoy Spooner

Michael Kenneth Kenrick

Nicholas Nun

Qingyan Au

Nicholas Hoe

Nam Tran

Aidan Mark Emery

Fiona Elizabeth Allan

Bonnie Lee Webster

Fiona Mary Ginty

Elizabeth Mary Collins

Antti Eljas Seppo

Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

Cell-free protein expression using rolling circle amplification product

Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/&#x3bc;L to about 35 ng/&#x3bc;L. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.

Cell-free protein expression using double-stranded concatameric DNA

The invention discloses a method for determining a nucleotide sequence of a nucleic acid segment present in a biological sample, comprising the steps of: a) generating a fluorescent image of the sample by a protocol comprising immunofluorescence detection of at least five different target proteins in the sample; b) selecting a region of interest of the sample by comparing the image to a predetermined criterion; c) removing a subsample from the region of interest, and; d) determining a nucleotide sequence of a nucleic acid segment present in the subsample.

Method of determining a nucleotide sequence of a nucleic acid segment from a region of interest in a biological sample

A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.

Photodegradable sample collection system and method

A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base, a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.

Extraction of materials from regions of interest in a sample

A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.

In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides

The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (PCR).

Methods for one step nucleic acid amplification of non-eluted samples

A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base; a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.

Methods and kits for DNA repair are provided. The methods and kits described herein repair multiple types of DNA damage. The kit may include a plurality of enzymes to repair a greater variety of lesions than any single enzyme is capable of repairing. Repair of damaged DNA may include releasing damaged bases from the DNA strand, nicking the DNA at the damaged sites, translating the nicks via 5'-3&#x2032; exonuclease activity, and sealing the nicks. The enzymes employed in the repair process may then be heat-inactivated, thereby obviating a purification process. The repaired DNA may then be analyzed using a variety of DNA analysis methods.

Method and kits for repairing nucleic acid sequences

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Clifton Park, NY, United States of America

Wei Gao