Dow Agrosciences LLC

Agrigenetics, Inc.

Wei Chen

Sandeep Kumar

Manju Gupta

Siva Prasad Kumpatla

Steven Robert Webb

Ning Zhou

Lakshmi Sastry-Dent

Nathan Joel VanOpdorp

Zehui Cao

Peter Friedemann

Matthew A Simpson

David Richard Corbin

Steve Joe Plehn

Peizhong Zheng

Stephen N Novak

Dayakar Reddy Pareddy

Thomas W Greene

Andrew F Worden

Ryan M Lee

Andrew Asberry

Yanxin Star Gao

Jafar Mammadov

Toby Cicak

Nathan Joel Van Opdorp

Nadia Chaidir

Heather Leigh Robinson

Tiffany King

Chandra-Shekara A Channabasavaradhya

Dennis Fitzl

Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

Reconstruction of site specific nuclease binding sites

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing aGRMZM2G144030 promoter. Some embodiments relate to aGRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to aGRMZM2G144030 3'UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

Plant promoter and 3'UTR for transgene expression

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing aGRMZM2G047720 promoter. Some embodiments relate to aGRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to aGRMZM2G047720 3'UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

DNA detection methods for site specific nuclease activity

The disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more floury2 (fl2) mutations. In embodiments, the disclosure concerns a gene specific PCR-based molecular (KASPar&#xae;) assay for identifying the floury2 trait in plants. In certain embodiments, compositions and methods are disclosed for determining the zygosity of corn plants with respect to the fl2 allele. In particular embodiments, the assay may be used for fl2 germplasm identification, accelerating introgression and molecular breeding programs in corn and other plants.

Floury 2 gene-specific assay in maize for floury (fl2) trait introgression

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from aUbiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.

Synthetic bi-directional plant promoter

This invention relates to methods for identifying maize plants that having increased green snap tolerance. The methods use molecular markers to identify and to select plants with increased green snap tolerance. Maize plants generated by the methods of the invention are also a feature of the invention.

Molecular markers associated with green snap in maize

This disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more brown midrib (BMR) mutations. The disclosure also concerns methods that are useful for enhancing the breeding process for BMR corn. In certain embodiments, compositions and methods for determining the zygosity of corn plants with respect to the bm3 allele.

Use of brown midrib-3 gene specific markers in maize for trait introgression

This disclosure concerns specific naturally-occurring mutant maize cad2 genes, which altered genes contribute to the bm1 maize phenotype in particular maize lines. In some embodiments, compositions and methods are provided that utilize a nucleic acid molecule comprising a mutant cad2 gene, and/or markers linked to a mutant cad2gene.

Gene and variations associate with BM1 phenotype, molecular markers, and their use

Growing community of inventors

Carmel, IN, United States of America

Wei Chen