The Dow Chemical Company

Wayne W Blaser

Mary Anne Leugers

Mark A LaPack

Kenneth R Beebe

Larry G Wright

A method of creating a library of standardized spectral data and identifying a sample by searching the standardized library for a match with the sample spectrum. The method entails determining and applying a transfer function which equalizes instrument bias between instruments and within the same instruments over time. A transfer function is specific to a particular instrument, and is based on the relationship between a reference spectrum of a standard as stored in a library and a spectrum of the standard obtained on the particular instrument of interest. This method provides enhanced library searching capabilities.

Method of creating standardized spectral libraries for enhanced library

A method of correcting for instrumental changes that influence the spectral response of a mass spectrometer over time. The method entails determining and applying a transfer function which equalizes instrument bias between mass spectrometers and within the same mass spectrometer over time. A transfer function is specific to a particular mass spectrometer, and is based on the relationship between a reference spectrum of a standard as stored in a library and a spectrum of the standard obtained on the particular mass spectrometer of interest. This method facilitates and simplifies recalibration, provides enhanced library searching capabilities for mass spectrometry, and may permit concentration determination from mass spectral data without the need to recalibrate an instrument with multiple standards.

Method of standardizing data obtained through mass spectrometry

A method is provided for uniformly coating a stationary liquid phase on glass beads, which are then suitable for use as chromatographic column packing, which comprises first contacting the beads with a first solution containing a suitable surfactant in an amount effective to promote the uniform deposition on the beads of the desired liquid phase, said surfactant being selected from the group consisting of (a) a nonionic surfactant, (b) an alkylaryl polyethylene ether phosphate, of the formula ##STR1## and (c) a mixture of nonionic surfactants, and then contacting the beads with a second solution containing a stationary liquid phase, the concentration of said stationary liquid phase in said second solution, the nature of the solvent in said second solution, and the conditions of said second contacting step being selected so that a coating of said statonary liquid phase of from about 0.05 to about 0.5 percent is imparted to said beads. Glass beads coated according to the above procedure represent an advance in the art of chromatography in that columns packed with these beads exhibit improved resolution and component adsorption characteristics.

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Midland, MI, United States of America

Wayne W Blaser