Mallinckrodt, Inc.

Wayne H Lin

Larry D Mosier

James J Grib

James W Woods

James L Brown

Disclosed are novel chemiluminescent compounds and their method of use in luminometric immunoassays. The compounds, comprising salts of phenanthridinium esters, are particularly useful in sandwich immunoassays.

Phenanthridinium ester as a labelling compound in luminometric

A double antibody radioimmunoassay method for determining antigens in a sample wherein polyethylene glycol is provided in a reaction medium to accelerate an immunoprecipitation reaction between water-soluble first antibodies bound to antigen and water-soluble second antibodies which are not specific to said antigens but which are specific to said first antibodies. A double antibody, multi-component radioimmunoassay test kit is also provided. The kit contains radiolabeled antigen reagent, first water-soluble antibody reagent and second water-soluble antibody reagent, as well as a polyethylene glycol reagent, which may optionally be combined with the second antibody reagent, in an amount sufficient to accelerate an immunoprecipitation reaction, between said first and second reagents.

Double antibody separation method

A method and reagent for counteracting lipemic interference in an assay of a lipid-containing sample, e.g. blood. The method includes the steps of: (a) mixing with the sample prior to or during said assay a high surface area, finely-divided adsorbent having an affinity for lipids, and a nonionic, water-soluble polymer, and (b) precipitating adsorbed lipids in said sample. The reagent includes a high surface area finely-divided adsorbent, and a nonionic, water-soluble polymer having a molecular weight of from about 1,000 to 100,000.

Method and reagent for counteracting lipemic interference

A container useful in immunochemical and enzymatical determinations or procedures.

Growing community of inventors

Chesterfield, MO, United States of America

Wayne H Lin