Arizona State University

Motorola Corporation

Wayne Frasch

Lars Chapsky

Fusheng Xiong

David Spetzler

Justin York

Herbert Goronkin

Frederic Zenhausern

Chia Fu Chou

Lyian He

The present disclosure relates to nucleic acid probes and kits for determining the length of a region of tandem repeats in a subject's genome and methods of using the DNA probes for determining the length of a region of tandem repeats in a subject's genome. In some embodiments, the region of tandem repeats in telomeres.

Probes and methods for measuring tandem repeats

The present disclosure relates to kits for determining the length of a region of tandem repeats in a subject's genome. In some embodiments, the region of tandem repeats in telomeres.

Methods, compositions, and kits for nucleic acid detection.

High speed, high fidelity, high sensitivity nucleic acid detection

A nanoscale motion detector attaches a gold nanorod () to the rotating arm () of a molecular structure () to cause the nanoparticle to rotate. The molecular structure is an F1-ATPase enzyme. The gold nanorod is exposed to a light source. The long axis of the gold nanorod scatters red light when the nanorod is in a first position. The short axis of the gold nanorod scatters green light when the nanorod is in a second position. A polarizing filter filters the red and green light to detect the rotational motion by observing alternating red and green lights. A detection DNA stand () is coupled between the gold nanorod and the molecular structure. The detection DNA strand hybridizes with a target DNA strand () if the target DNA strand matches the detection DNA strand to form a structural link between the molecular structure and gold nanorod.

Polarization-enhanced detector with gold nanorods for detecting nanoscale rotational motion and method therefor

The present invention provides methods for generating a distribution of optimal answers to a nondeterministic polynomial optimization problem by providing a plurality of solutions comprising input polynucleotides, wherein each solution comprises identical input polynucleotides; and wherein the number of solutions comprising polynucleotides equals a number of data inputs in the problem to be answered, and wherein each input polynucleotide comprises an x segment and a y segment; providing a plurality of solutions comprising connection polynucleotides wherein each solution comprises identical connection polynucleotides; and wherein the number of solutions comprising connection polynucleotides equals a number of unique connections that can be made between the different data inputs, and wherein each polynucleotide in the set of connection polynucleotides is complementary to the x segment of one input polynucleotide and to the y segment of one different input polynucleotide; combining the solutions comprising the input polynucleotides with the solutions comprising the connection polynucleotides to form a hybridization mixture, wherein the combining is done under conditions to promote formation of hybridization complexes between complementary polynucleotides, and wherein each individual connection polynucleotide is added at a concentration based on a weighted value assigned to the individual connection polynucleotide; ligating the polynucleotides that are present in the hybridization complexes to form ligation products; and determining a concentration of the ligation products, wherein the ligation products present at the highest concentration represent optimal answers to the nondeterministic polynomial optimization problem.

Methods for generating a distribution of optimal solutions to nondeterministic polynomial optimization problems

The present invention provides methods, compositions, and kits for nucleic acid detection.

The present invention provides methods and compositions for highly sensitive nucleic acid detection, down to the single nucleic acid molecule level. In one aspect, the present invention provides methods for detecting a target nucleic acid comprising: (a) providing first and second target-specific nucleic acids, wherein the first and second target-specific nucleic acids each comprise sequences complementary to the target nucleic acid; wherein the first target specific nucleic acid is bound to a first affinity tag and the second target-specific nucleic acid is bound to a second affinity tag, wherein the first affinity tag is capable of binding to a molecular motor, and wherein the second affinity tag is capable of binding to a detection probe; (b) contacting the first and second target-specific nucleic acids to a sample under conditions whereby the first and second target-specific nucleic acids will hybridize to the target nucleic acid if the target nucleic acid is present in the sample, wherein upon hybridization to the target nucleic acid the first and second target-specific nucleic acids are directly adjacent to each other; (c) ligating the first and second target-specific nucleic acids together; (d) binding the molecular motor t&#x2dc; the first affinity tag and the detection probe to the second affinity tag; (e) inducing movement of the molecular motor; and (f) detecting movement of the molecular motor through the detection probe, wherein the movement of the molecular motor serves to detect the target nucleic acid in the sample.

Single molecule detection using molecular motors

An exemplary system and method of employing DNA hybridization for the detection of bio-agents is disclosed as comprising inter alia a biomolecular rotary motor (); a capture probe DNA fragment () effectively attached to said biomolecular motor (); a target DNA fragment () suitably adapted for hybridization with said capture probe DNA (); a signal probe DNA fragment () suitably adapted for hybridization with said target DNA (); and a fluorescent bead () attached to said signal probe DNA (). Disclosed features and specifications may be variously controlled, adapted or otherwise optionally modified to improve certain device fabrication parameters and/or performance metrics.

Growing community of inventors

Phoenix, AZ, United States of America

Wayne Frasch