Zeteo Tech, Inc.

Johns Hopkins University

Veridian Erim International, Inc.

Wayne A Bryden

Michael McLoughlin

Dapeng Chen

Charles John Call

Thomas McCreery

Scott A Ecelberger

Peter F Scholl

Michael P McLoughlin

Micah A Carlson

Charles W Anderson

Scott Ecelberger

Steven Strohl

Nathaniel K Jones

Emily Caton

Harvey Wayne Ko

Robert J Cotter

Richard S Potember

Timothy J Cornish

William R Allmon

Charles E Daitch

James T Velky

Nicholas H Evancich

Daniel J DeCicco

Ross Kliegman

Eric Black

Gary Anderson

Jonathan Nusbaum

Lara Moore

Ronald G Chappell

Thomas Mccreery

Disclosed are methods and devices for analyzing aerosol particles in exhaled breath using diagnostic tools that enable rapid, low cost and autonomous point of care assays for several diseases including respiratory tract diseases. Disclosed are methods and devices for capturing exhaled breath aerosols in a packed bed column and analyzing exhaled captured breath aerosols for tuberculosis diagnosis.

Diagnosis of tuberculosis and other diseases using exhaled breath

Methods and devices for analyzing non-volatile organics in exhaled breath and other aerosols using various diagnostic tools that enable rapid, low-cost point of care assays for several diseases including respiratory tract diseases such as COVID-19. The disclosed methods and systems selectively capture non-volatile organics in exhaled breath and other aerosols in a packed bed column. The non-volatile organics are eluted and samples are analysis using diagnostic devices including MALDI-TOFMS. The disclosed systems and methods provide for a diagnostic test result in less than about 20 minutes and provides for autonomous operation with minimal human intervention.

Diagnosis of respiratory diseases using analysis of exhaled breath and aerosols

In-vehicle occupant detection system and methods capable of monitoring the interior of a passenger vehicle and responding to potentially dangerous situations are disclosed. The detection systems may comprise a front camera to focus on driver and passenger behavior in the front cabin, and a rear camera and an array of environmental sensors to detect unattended occupants including children, adults or pets in the rear seats. Data may be processed by an intelligent algorithm to assesses anomalies and trigger one or more alarms or countermeasures.

In-vehicle occupant safety monitoring systems and methods

Mobile decontamination systems and microwave assisted decontamination methods for decontaminating a variety of contaminated surfaces disposed external to the system. The systems and methods include treating the surfaces with benign chemical formulations followed by exposing to microwave irradiation for short periods of time to achieve at least 6-log reduction in biological contaminants including viruses and spores of, and. The formulations include a percarbonate and surfactant in water.

Mobile systems for microwave assisted surface decontamination and decontamination methods

Systems and methods to provide rapid and autonomous detection of biological and chemical analyte particles in gas and liquid samples. Systems and methods for capturing and identifying biological and chemical aerosol analyte particles using matrix assisted laser desorption mass spectrometry (MALDI-MS) and using time-of-flight mass spectrometry (TOFMS) are disclosed. High specificity for capture and detection of aerosolized fentanyl was demonstrated using a portable sample capture and analysis system.

Systems and methods of rapid and autonomous detection of aerosol particles

Disclosed are systems are methods for identifying the composition of single aerosol particles, particularly that of bioaerosol particles, without pre-treatment using complex organic MALDI matrices. A continuous timing laser may be used to index aerosol particles, measure particle properties, and trigger a pulse ionization laser. Ionized fragments and optionally photons associated with each particle producing by the ionization laser may be analyzed using one or more detectors including a TOF-MS detector and an optical detector. The laser pulse may comprise a simultaneous IR and UV laser pulse when fragments comprise predominantly of UV chromophores. Unique spectral data associated with each indexed particle from each detector may be compiled using data fusion to generate compiled spectral data. Machine learning methods may be used to improve the prediction of composition over time.

Methods and systems for detecting aerosol particles without using complex organic MALDI matrices

Mobile decontamination systems and microwave assisted decontamination methods for decontaminating a variety of contaminated surfaces disposed external to the system are disclosed. The systems and methods comprise treating the surfaces with benign chemical formulations followed by exposing to microwave irradiation for short periods of time to achieve at least 6-log reduction in biological contaminants including viruses and spores of, and. The formulations comprise a percarbonate and surfactant in water.

Disclosed are microwave assisted methods and systems for decontaminating a variety of contaminated surfaces. The systems and methods comprise treating the surfaces with benign chemical formulations followed by exposing to microwave irradiation for short periods of time to achieve at least 6-log reduction in biological contaminants including spores of, and. Chemical formulations may comprise copper (II) chloride in water. The formulations may include a surfactant such as a polyethylene sorbitol ester surfactant.

Microwave assisted methods and systems for surface decontamination

Disclosed are methods and devices for analyzing non-volatile organics in exhaled breath and other aerosols using various diagnostic tools that enable rapid, low cost point of care assays for several diseases including respiratory tract diseases such as COVID-19. The disclosed methods and systems selectively capture non-volatile organics in exhaled breath and other aerosols in a packed bed column. The non-volatile organics are eluted and samples are analysis using diagnostic devices including MALDI-TOFMS. The disclosed systems and methods provide for a diagnostic test result in less than about 20 minutes and provides for autonomous operation with minimal human intervention.

Disclosed are systems and methods to provide rapid and autonomous detection of analyte particles in gas and liquid samples. Disclosed are methods and devices for identifying biological aerosol analytes using MALDI-MS and chemical aerosol analytes using LDI and MALDI-MS using time-of-flight mass spectrometry (TOFMS).

Coating materials such as MALDI matrix solutions are aerosolized and are used to coat analyte particles in an acoustic coater. Methods and devices for coating analyte particles in real time are disclosed. The coating improves the detection and quantification of the analyte particles using analytical instruments such as an aerosol time of flight mass spectrometer.

Coating of aerosol particles using an acoustic coater

A method and apparatus is described for neutralizing airborne pathogens in ventilated air, and in heating or air conditioning systems. The pathogen neutralization system is effective against a wide spectrum of pathogens, it incorporates commercially available components, and it can be readily integrated into commercial HVAC systems where it neutralizes airborne pathogens in large volumes of ventilated air in real time without any chemical reagents. Typically, the system has a flow-through reaction chamber that contains a UV light source that emits short intense flashes of broad-spectrum UV light, a source of water vapor or spray, and an ozone generator. The system generates highly reactive ozone intermediates by irradiating ozone gas with UV light in the presence of water droplets or water vapor. The pathogens that can be neutralized by this system include bacteria, viruses, spores, fungi and parasites.

Growing community of inventors

Ellicott City, MD, United States of America

Wayne A Bryden