Mitsubishi Tanabe Pharma Corporation

Mitsubishi Pharma Corporation

Wataru Otani

Masami Miura

Masaaki Hirose

Hiroyuki Irie

Shinobu Kuwae

Kenmi Miyano

Taeko Miwa

Hideyuki Ohi

A protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus; a gene coding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genushaving been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced, are described.

Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby

A gene participating in addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genusis described. Also described is a means of suppressing a functional product encoded by the gene.

The present invention intends to find out a gene participating in addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genusand provide a means of controlling the same. The present invention also intends to provide a process for producing a protein with reduction of an acidic sugar chain by using the thus controlled yeast strain belonging to the genus. Namely, the present invention includes a protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein; a gene encoding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genushaving been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced.

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Tokyo, Japan

Wataru Otani