Mitsubishi Chemical Corporation

Mitsubishi Rayon Company, Limited

Nitto Chemical Industry Co., Ltd.

University of California

Wataru Mizunashi

Fujio Yu

Eiji Sato

Tetsuji Nakamura

Takanori Akiyama

Eiji Nakajima

Michiko Yamazaki

Yasuhisa Asano

Takakazu Endo

Yoshihiro Hashimoto

Makoto Kaneko

Kevin V Solomon

Michelle O'Malley

Mami Kato

Toshio Fujita

The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genusor

Method for producing methacrylyl-CoA

It is intended to provide an efficient method for expressing a protein of interest as an active soluble recombinant protein in a transformant. The present invention provides a method for producing an enzyme having improved activity per recombinant as compared with a reference form, comprising the steps of: (1) preparing recombinants each expressing a mutant having an amino acid sequence derived from the amino acid sequence of the reference form by the substitution of one or more cysteine residues by other amino acid residues; (2) selecting a plurality of mutants that exhibit 50% or more activity per recombinant relative to 100% activity of the reference form; and (3) expressing a mutant in which corresponding amino acid residues are substituted at two or more positions among the respective positions of the substituted amino acid residues of the mutants selected in step (2).

Method for producing mutant enzyme, and mutant alcohol acyltransferase

Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step for treating methacrylyl-CoA with an alcohol or phenol in the presence of an alcohol acyltransferase to synthesize the methacrylic acid ester. According to this production method, a methacrylic acid ester can be efficiently produced while largely reducing energy, resource and environmental load, compared with the conventional chemical production processes.

Method for producing methacrylic acid ester

The invention relates to an engineered eukaryotic microorganism into which a gene encoding an acyl-CoA dehydrogenase is introduced and a method for producing methacrylic acid esters such as MMA and MMA-CoA and precursors thereof using the microorganism.

Biological production of methyl methacrylate

Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step of reacting an alcohol or a phenol with methacrylyl-CoA in the presence of an alcohol acyltransferase originated from a plant selected from the group consisting of a plant belonging to the genus, a plant belonging to the genus, a plant belonging to the genus, a plant belonging to the genus, a plant belonging to the genusand a plant belonging to the genusto thereby synthesize the methacrylic acid ester.

Method for producing methacrylic acid ester and novel methacrylic acid ester synthetase

To provide a method for directly and efficiently producing methacrylic acid in a single step from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials. Further provided is a method for producing methacrylic acid using microbes having the ability to produce methacrylic acid, from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials, as a carbon source and/or energy source. The method for producing methacrylic acid enables methacrylic acid to be safely and easily produced from biomass, without using petroleum-derived raw materials, by utilizing microbes having the ability to produce methacrylic acid.

Method for producing methacrylic acid and/or ester thereof

The present invention provides a modified ethylenediamine-N,N'-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N&#x2032;-disuccinate:ethylenediamine lyase activity.

Modified ethylenediamine-N,N'-disuccinate:ethylenediamine lyase

Modified ethylenediamine-N, N'-disuccinate: ethylenediamine lyase

Provided are a DNA fragment containing a gene relating the autonomous replication of a plasmid, and a DNA fragment containing a gene relating to the autonomous replication of a plasmid wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene. Also provided are a DNA fragment containing a gene having a function relating to the autonomous replication of a plasmid within the bacteria of the genus, a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene, and a plasmid carrying a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site of the gene.

DNA fragments containing gene having function relating to autonomous proliferation of plasmid

The present specification relates to a DNA comprising a nucleotide sequence coding for a polypeptide having ethylenediamine-N,N&prime;-disuccinic acid:ethylenediamine lyase activity, the polypeptide having the amino acid sequence of SEQ ID NO: 1 which may have a deletion, substitution or addition of at least one amino acid; a recombinant plasmid containing the DNA; a host transformed with the recombinant plasmid; and a method for producing a diaminoalkylene-N,N&prime;-disuccinic acid using the transformed host. The present specification also relates to a polypeptide having ethylenediamine-N,N&prime;-disuccinic acid:ethylenediamine lyase activity which has the amino acid sequence of SEQ ID NO: 1 that may have a deletion, substitution or addition of at least one amino acid.

Protein having ethylenediamine-N,N&prime;-disuccinic acid:ethylenediamine lyase acitivity and gene encoding the same

A process for producing an optically active aminopolycarboxylic acid, such as S,S-ethylenediamine-N,N'-disuccinic acid, from a mixture of a diamine, such as ethylenediamine, with fumaric acid using a microorganism having a lyase activity, wherein at least one metal ion selected from the group consisting of an alkaline earth metal, iron, zinc, copper, nickel, aluminum, titanium and manganese is added to the reaction system. According to this process, aminopolycarboxylic acids, such as S,S-ethylenediamine-N,N'-disuccinic acid, or metal complexes thereof, can be appropriately and efficiently produced while improving the reaction yield.

Process for producing optically active aminopolycarboxylic acid

The specification relates to circular plasmids derived from microorganisms of the genus Rhodococcus, which have sizes of about 3.6 kb and restriction site numbers of BamHI:1, BglII:1, ClaI:1, PstI:1, PvuII:2 and SacI:1. The circular plasmids of the present invention are useful as vectors in industrially useful host-vector systems.

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Kanagawa, Japan

Wataru Mizunashi