Nitto Boseki Co., Ltd.

Wataru Kikuchi

Kenta Noda

Ryo Kojima

Toshihide Miura

Fumio Nomura

Iwao Kiyokawa

Kazuyuki Sogawa

Kumiko Sasagawa

Momoe Sato

The purpose of the present invention is to provide a monoclonal antibody that is useful in specifically assaying tartrate resistant acid phosphatase 5b (TRACP-5b). A hybridoma producing a monoclonal antibody against TRACP-5b, said monoclonal antibody showing higher reactivity with TRACP-5b than with tartrate resistant acid phosphatase 5a (TRACP-5a) and, therefore, being specific to TRACP-5b, is obtained by cell fusion using, as an antigen, human recombinant TRACP-5b purified from silkworm silk gland. By using this monoclonal antibody, TRACP-5b in a specimen can be highly sensitively and specifically detected.

Protein assay method specific to TRACP-5b (tartrate resistant acid phosphatase 5b)

Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the &#x3b1;-E chain and &#x3b1; chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.

5.9 kDa peptide immunoassay method

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Koriyama, Japan

Wataru Kikuchi