University of Minnesota

Other

University of Toronto

Warren Chan

John Bischof

Taner Akkin

Zhenpeng Qin

Li Zhan

Hans Christian Fischer

Steven Perrault

Sawitra Mardyani

Wen Jiang

Leo Chou

John C Bischof

Assays used in conjunction with a thermal contrast reader are disclosed. In the assay, the test strip includes materials that can develop a thermal response if a target analyte is present in a sample. Linear flow assays include nanoparticles with high affinity binding to the analyte. Binding of the nanoparticles with an analyte in the sample is detected using thermal contrast. Analytes over a broad range of concentrations are detected in the linear flow assays. Methods of detecting target analytes and kits comprising lateral flow assays and thermal contrast reader are also disclosed.

Lateral flow assays with thermal contrast readers

Assays used in conjunction with a thermal contrast reader are disclosed. In the assay, the test strip includes materials that can develop a thermal response if a target analyte is present in a sample. The thermal contrast reader includes housing having an opening to receive the test strip at a test location, an energy source directed at the test location and a heat sensor directed at the test location. The heat sensor is configured to sense heating of the test strip upon activation of the heat source at the test location, if the target analyte is present in the sample. The heat sensor can provide sensor output using diagnostic circuitry coupled to the sensor output and configured to provide a diagnostic output. The diagnostic output can indicate the diagnostic condition of the patient as a function of the sensor output. The present disclosure also includes methods of detecting target analytes and kits comprising lateral flow assays and thermal contrast reader.

Thermal contrast assay and reader

A process for the production of fluorescent nanoparticles selected from noble metal or silica nanoparticles. Noble metals or silica nanoparticles provide a platform to which fluorophores and other detection molecules can be added. The fluorescent nanoparticles can be used as regents, diagnostic assays and in the tracking or targeting of tumors.

Synthesis of fluorescent noble metal nanoparticles

A method for manufacturing powdered quantum dots comprising the steps of: a) reacting quantum dots comprising a core, a cap and a first ligand associated with the outer surfaces thereof with a second ligand, the second ligand displacing the first ligand and attaching to the outer surfaces of the quantum dots, b) isolating the quantum dots having the attached second ligand from the reaction mixture, c) reacting the isolated quantum dots having the attached second ligand with a small organic molecule whereby the small organic molecule attaches to the second ligand, d) reacting the quantum dots having the attached small organic molecule with a cross-linking agent to cross-link the small organic molecule attached to the second ligand with an adjacent second ligand attached to the surfaces of the quantum dots, e) isolating the quantum dots formed in step (d); and f) drying the isolated quantum dots to form powdered quantum dots. The invention includes the quantum dots.

Stable, water-soluble quantum dot, method of preparation and conjugates thereof

Growing community of inventors

Toronto, Canada

Warren Chan