Industrial Technology Research Institute

Wann-Hsin Chen

Chen-Ming Chen

Jinn-Fa Wu

Yeou-Bin Guu

Yu-Hsi Hsing

Lih-Tao Hsu

Su-Yo Lin

Polynucleotides associated with differentiation states of stem cells are provided. Also provided are methods and kits for detecting, identifying and/or discriminating differentiated stem cells from undifferentiated ones by measuring an expression level of one or more genes, such as CBARA1 and LHX 6, in the stem cells.

CBARA1 and LHX6 cell markers for embryonic stem cells

A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the base, and on the platform is defined with the holder for receiving a plurality of loading trays, so as to the original cell colonies and a plurality of media boards of subculture can be placed on the loading trays of holder, the holder can displace from a first axis feeding means and a second axis feeding means of feeding mechanism to moveably align with a first axis and a second axis. In addition, the cutting means and the transplanting means respectively capable of vertically move along a third axis feeding means of feeding mechanism are arranged onto the base, wherein the cutting means is provided with an array of invertable blades, and the transplanting means is defined with a bent capture needle for capturing the cell masses, whereby enabling to use operational analysis of controller to precisely cut, capture and pickedly place the original cell colonies.

Cell colonies dissecting and transplanting apparatus

Methods are provided for producing cardiomyocytes suitable for various medical applications. In one embodiment, a method of producing cardiomyocytes is provided, which comprises culturing mesenchymal stem cells in a medium containing ascorbic acid. In another embodiment, such method further comprises the step of selecting fast-growing mesenchymal stem cell clones before culturing the selected clones in the culturing medium that contains ascorbic acid.

Growing community of inventors

Hsinchu, Taiwan

Wann-Hsin Chen