Capitalbio Corporation

Tsinghua University

Capitalbio Technology Corporation

Sichuan University

Capitalbio Technoloogy Corporation

Capitalbiochip Corporation

Wanli Xing

Jing Cheng

Guangxin Xiang

Lei Wang

Dong Wang

Guoqing Wang

Guoliang Huang

Guanbin Zhang

Jiaming Ye

Di Jiang

Mingxian Lin

Guohao Zhang

Pinhong Wang

Can Wang

Yang Li

Jing Zhu

Xiang Chen

Dong Liang

Yao Zhou

Hu Wang

Xianhua Wang

Huafang Gao

Liang Bai

Kaijun Zhao

Xinying Zhou

Zhongyao Yu

Liangbin Pan

Hongju Guo

Feijun Xian

Jian Gao

Peng Liu

Shuo Li

Hui Wang

Liang Zhang

Yao Zhang

Rui Ma

Yan Zhang

Yang Wang

Li Li Ma

Jia Wang

Lan Xie

He Yan

Jiang Zhu

Yuxiang Zhou

Ying Lu

Juan Xin

Yuming Hu

Tao Guo

Xiaolei Zhao

Yimin Sun

Zhe Li Yu

Baolian Li

Tengfei Guo

Tian Qiu

Renyuan Chen

Fei Jun Xian

Bin Zhuang

Zixin Zhang

Xianbo Luo

Zhengde Feng

Kaihuan Zhang

Tao Sheng

Shuang An

Tongjun Wang

Su Guo

Liqing Duan

Jinxiu Zhang

Xiumei Song

Xueling Quan

Lihua Huang

Xianhua Tsinghua University Wang

A method of enriching nucleic acid, including mixing a sample with a solid phase extraction material; and separating the solid phase extraction material; wherein the solid phase extraction material is glass beads or magnetic beads modified with reduced graphene oxide. Also disclosed is a method of detecting nucleic acid, including mixing a nucleic acid sample enriched by the method above with a probe; and amplifying and detecting an amplification product by electrophoresis.

Solid phase extraction material and its use for nucleic acid enrichment and detection

A liquid storage and controlled-release device and a biological detection chip. The liquid storage and controlled-release device comprises a liquid storage capsule with a liquid storage body which is deformable under a pressure, and a sealing layer for sealing the liquid storage body. A support platform is provided right below the liquid storage capsule and tightly connected with the liquid storage capsule, wherein, a directional release chamber is provided at the middle of the support platform, and the directional release chamber is provided with a guiding chamber for collecting the liquid and a sharp edge for inducing break. Compared with the conventional technology, the opening for liquid release is at the end far away from the rotation center, so that the liquid can be released completely by the centrifugal force, ensuring the quantitative release of stored liquid and reducing the influence on the accuracy of the subsequent detection.

Liquid storage and controlled-release device and biological detection chip

An integrated microfluidic chip, wherein at least one integrated reaction unit is provided on its substrate, and the integrated reaction unit comprises at least a sample cell (), a mixing cell () and a reaction cell () connected through liquid channels (). In one aspect, one end of the sample cell () is provided with a sample inlet (), and the chip further comprises an internal air circulating system/circuit. One end of the internal air circulating system/circuit is connected with the mixing cell (), while the other end comprises at least a first circulation branch circuit connected with the end of the sample cell () distal to the sample inlet ().

Integrated microfluidic chip and methods of use

In one aspect, the present disclosure provides an integrated microfluidic device for nucleic acid amplification and microarray detection. In one aspect, the device comprises: (1) a microchip configured to process reagents, comprising a plurality of reservoirs, channels, valves, and/or fluid interfaces; (2) an amplification chamber for PCR, carried out in a detachable tube assembled on the microchip through a joint; and (3) a microarray chamber comprising a microarray and a reaction chamber. In some embodiments, these features are interconnected to allow transportation of reagents for nucleic acid amplification and hybridization detection functions in a closed system. In one aspect, the integrate device herein overcomes the problem of contamination during the amplification and hybridization reactions.

Integrated microfluidic device for target amplification and microarray detection

A microfluidic valve provided herein is configured to mix or capable of mixing a sample and/or a reagent in addition to controlling liquid flow. In one embodiment, the microfluidic valve comprises a rotor () and one or more micro-structures () that move with the rotation of the rotor (). In one embodiment, the one or more micro-structures () stir and/or mix content in a mixing chamber () formed by the rotor (), a base (), and a sleeve () of the microfluidic valve. A microfluidic chip or chip system comprising one or more of the microfluidic valves, and methods of use, are also provided.

Microfluidic valve and a chip or system comprising the microfluidic valve

A method for labeling target molecules coupled to particles for the detection of the target molecules using a microarray chip, comprises: providing a functionalized microparticle, wherein the microparticle is coated with one or more functional group; providing a modification group on each of the target molecules to be detected to form modified target molecules; contacting the functionalized microparticle with the modified target molecules; coupling a luminophore to the complex between the functionalized microparticle and the modified target molecules, thereby directly or indirectly labeling each modified target molecules with the luminophore. By directly or indirectly labeling the target molecules with the luminophore, the method reduces the cost of fluorescence detection, and avoids PCR inhibition derived from traditional fluorescence labeling molecules.

Luminophore-labeled molecules coupled with particles for microarray-based assays

In one aspect, a microfluidic device for multiple reactions is provided, which comprises a reaction channel comprising multiple reaction chambers connected to a closed chamber or an elastic balloon outside of the microfluidic device, wherein a wall of the closed chamber is an elastic membrane; and a control channel comprising an elastic side wall, wherein the intersections between the side wall of the control channel with the reaction channel form multiple pneumatic microvalves. In another aspect, a method for conducting multiple reactions using the microfluidic device is provided, which comprises: a) filling the reaction chambers with a sample; and b) applying pressure to the control channel to expand the elastic side wall of the control channel, wherein the expanded elastic side wall forms a pneumatic microvalve that separates the reaction chambers.

Microfluidic devices for multi-index biochemical detection

Provided herein is a multi-sample and multi-locus method for analyzing a genetic locus. In particular, provided herein is a method for SNP detection and analysis based on high-throughput sequencing, comprising designing a probe, pre-amplification and biotin labeling, hybridization, ligation, barcode specific primer extension, sequencing and analyzing the SNP locus. A probe set is for the analysis is also provided.

Detection methods based on sequencing

Isothermal amplification microfluidic chip nucleic acid analyzer

A multi-index detection microfluidic chip is provided. A bottom plate () and a cover plate () that matches the bottom plate () and seals it are provided in the microfluidic chip. The center of the microfluidic chip has a through-hole (). The bottom plate () has one or more wave-shaped main channel(s) (), one end of each of the main channel () connected to a sample injection hole () on the bottom plate (), and the other end connected to an exhaust hole () on the bottom plate (). The valley on the main channel () is distal to the through-hole (), and the peak is proximal to the through-hole (). Each valley of the main channel () is connected to a reaction chamber () by a linking channel (). The linking channel () comprises one or more buffering chambers (). The microfluidic chip can be used in detection by fluorescence, turbidity, color, detection equipment, and/or direct observation by the naked eyes. The detection is real-time as the reaction occurs or after the reaction.

Multi-index detection microfluidic chip and methods of use

The present disclosure provides a cover sheet for a microarray reaction device. In one aspect, the present cover sheet or device ensures the reaction units/volumes are stable and/or consistent among assay samples and assay runs, allowing samples (e.g., reaction solutions) to be conveniently added and distributed uniformly.

Micro-reactor array

Provided is a microfluidic chip, which comprises a substrate and a cover sheet, wherein a microreactor array is arranged on the substrate and comprises at least one main channel () and at least two micro cells () connected to the main channel (). The microfluidic chip also comprises at least one local temperature control device, which is used for heating the main channel () or cooling the micro cells (). The use of the microfluidic chip ensures uniformity and independency of the micro cells (). Also provided is an application of the microfluidic chip in biological detection or medical inspection.

Microfluidic chip and application thereof

A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises coupling a target molecule to a particle and then binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. Coupled with microarray, the particles, themselves or further modified, facilitate the detection of results with non-expensive devices or even naked eyes. This technology enables the detection and interpretation of molecular interactions in an efficient and cost effective way.

Microarray-based assay integrated with particles for analyzing molecular interactions

A hybridization system is provided, which comprises a biological chip having a substrate () with a probe dot matrix () and a cover plate () with at least two through-holes (), where a hybrid chamber () is formed between the substrate () and the cover plate (), at least two fluid channels () are interconnected respectively with the hybrid chamber () by the two through-holes (), and a fluid control device is interconnected with the fluid channels (). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels () and the chamber () to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.

Biological chip hybridization system

Growing community of inventors

Beijing, China

Wanli Xing