Zhejiang University

Wanjie Dong

Xu Liu

Cuifang Kuang

Shaocong Liu

Disclosed is a phase-modulated fluorescence differential microscopic imaging device based on electro-optical modulation technology, including an illumination system for generating an excitation beam, a detection system for collecting a fluorescent signal emitted by a sample, and a computer for controlling and signal processing. The illumination system includes the following components that are sequentially arranged: a laser, emitting a laser beam; an electro-optical modulation module, configured to control a rapid conversion of the laser beam between a right-handed circularly polarized light and a left-handed circularly polarized light; and a phase modulation device, configured to modulate a circularly polarized light to form a solid light spot and a hollow light spot for illuminating the sample and exciting fluorescence. Also disclosed is a phase-modulated fluorescence differential microscopic imaging method based on the electro-optical modulation technology, which realizes a rapid conversion of a solid light spot and a hollow light spot, and improves the imaging speed of the fluorescence differential microscopic imaging system to a large extent.

Growing community of inventors

Hangzhou, China

Wanjie Dong