Monsanto Technology LLC

Wanggen Zhang

Brenda A Lowe

Jyoti R Rout

Michael T Mann

David R Duncan

Anisha Akula

William L Petersen

David D Songstad

Joel B Wilks

Larry A Gilbertson

Xudong Ye

Elysia K Krieger

Elysia Krieger

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Methods for producing transgenic plants

Production of transformed plants is achieved by placing-inoculated explants directly onto a medium containing selective agents for suppressing growth ofand killing non-transformed explant cells.

The present invention has incorporated a non-lethal negative selectable marker gene into the vector backbone DNA of a DNA plasmid used to transform plant cells. These transgenes are designed to express a non-lethal gene product in plant cells that contain the vector backbone DNA of the DNA plasmid. The gene products of the non-lethal negative selectable marker gene are involved in plant hormone biosynthesis pathways, plant hormone substrate diversion, plant hormone degradation, plant hormone signaling or metabolic interference. The use of these DNA plasmids to transform plant cells provides for the enhanced production of commercially viable plants.

DNA constructs and methods to enhance the production of commercially viable transgenic plants

Growing community of inventors

Wildwood, MO, United States of America

Wanggen Zhang