Metabolic Explorer

Evonik Degussa Gmbh

Metalbolic Explorer

Wanda Dischert

Rainer Figge

Philippe Soucaille

Perrine Vasseur

Laurence Dumon-Seignovert

Zhen Chen

Cédric Boisart

Céline Raynaud

Gwénaëlle Bestel-Corre

Gwenaëlle Corre

An-Ping Zeng

Nadège Dumoulin

Feng Geng

Guillaume Letellier

Véronique Douchin

The present invention concerns a new mutant strain ofcomprising attenuated glycerol kinase activity. In addition, the present invention concerns a consortium ofcomprising at least said mutant strain and at least one other species ofchosen amongand. As this modified strain may be adapted for growth and for the production of 1,3-propanediol in an appropriate culture medium with high glycerol content, the invention also relates to a method for the production of 1,3-propanediolandbutyric acid, by culturing at least this mutant strain in an appropriate culture medium.

Microorganisms with improved 1,3-propanediol and butyric acid production

The present invention relates to a recombinant microorganism capable of producing 2,4-dihydroxybutyrate, which is characterized by an increased cellular export, and preferably by a decreased cellular import, of said 2,4 DHB. The invention also relates to a method for the optimized production of 2,4-dihydroxybutyrate by culturing said microorganism in a fermentation medium and recovering 2,4-DHB from said medium.

Modified microorganism for the optimized production of 2,4-dihydroxybutyrate with enhanced 2,4-dihydroxybutyrate efflux

The present invention relates to methods and recombinant microorganisms useful for the production of glycolic acid and/or glyoxylic acid. The methods of the invention involve either only one step of fermentation of the modified microorganisms of the invention or one step of fermentation of the modified microorganisms of the invention and one step of either biological or chemical conversion of the glycolic acid or of the glyoxylic acid, the microorganism of the invention over expressing a phosphoketolase gene.

Methods and microorganisms for the production of glycolic acid and/or glyoxylic acid

The present invention concerns a method for the production of derivatives of 4-hydroxy-2-ketobutyrate chosen among 1,3-propanediol or 2,4-dihydroxybutyrate by culturing a genetically modified microorganism for the production of the desired derivative of 4-hydroxy-2-ketobutyrate, the microorganism further comprising a gene coding for a mutant glutamate dehydrogenase converting by deamination L-homoserine into 4-hydroxy-2-ketobutyrate. The invention also concerns said genetically modified microorganism.

Mutant glutamate dehydrogenase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate

The present invention is related to a recombinant microorganism optimized for the fermentative production of methionine and/or its derivatives, wherein in said recombinant strain, the methionine efflux is enhanced by overexpressing the homologous logous genes of ygaZ and ygaH genes from. It is also related to a method for optimizing the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine efflux is enhanced by overexpressing the ygaZH homologous genes of ygaZ and ygaH genes from, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and/or its derivatives from the culture medium.

Method and microorganism for methionine production by fermentation with improved methionine efflux

The present application is related to a recombinant microorganism optimized for the fermentative production of methionine and/or its derivatives, wherein in said recombinant microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced. The application is also related to a method for optimizing the fermentative production of methionine and/or its derivatives comprising the steps of: c. culturing a recombinant microorganism wherein in said microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and d. recovering methionine and/or its derivatives from the culture medium.

Microorganism for methionine production with improved methionine synthase activity and methionine efflux

The present invention is related to a recombinant microorganism for improved methionine production comprising modifications to produce methionine from glucose as main carbon source by fermentation, and modifications to improve glucose import, wherein the glucose import is improved by modifying the expression of at least one gene selected from ptsG, sgrT sgrS and dgsA. The invention is also related to a method for the fermentative production of methionine or methionine derivatives comprising the steps of:

Microorganism for methionine production with enhanced glucose import

The present invention is related to a recombinant microorganism optimized for the fermentative production of methionine, wherein the activity of the cobalamin-independent methionine synthase MetE is attenuated in said microorganism. The invention is also related to a method for producing methionine by fermentation.

Recombinant microorganism for the fermentative production of methionine

The present invention relates to use of inducible promoters in the production of glycolic acid by fermentation. The present invention concerns a method for the production of glycolic acid in a fermentative process comprising the following steps: The invention also concerned the modified microorganism used in the method of glycolic acid production.

Method for producing glycolic acid using an inducible promoter

The present invention is related to a microorganism for the production of methionine, wherein said microorganism is modified to enhance the transhydrogenase activity of PntAB. In a preferred aspect of the invention, the activity of the transhydrogenase UdhA is attenuated in said microorganisms. The invention also related to a method for producing methionine by fermentation.

Increasing NADPH availability for methionine production

The present invention is related to a method for the fermentative production of hydroxymethionine, comprising the steps of: In a specific embodiment, the recombinant microorganism is cultivated under conditions of nitrogen limitation. The invention is also related to the biologically-produced hydroxymethionine and its uses.

Fermentative production of methionine hydroxy analog (MHA)

The present invention relates to an improved method for the bioconversion of a fermentable carbon source to glycolic acid by a recombinant microorganism bearing new genetic modifications such as &#x394;ldhA, &#x394;mgsA, &#x394;arcA, and &#x394;lldP, &#x394;glcA, &#x394;yjcG and combination of them allowing a production with higher yield, titer and productivity.

Growing community of inventors

Vic-le-Comte, France

Wanda Dischert