Boehringer Mannheim Gmbh

Walter W Jernigan

Christopher Douglas Wilsey

Gerald F Sigler

Nigel A Surridge

Zhi David Deng

Robert J McEnroe

Rebecca W Muddiman

Chi-Neng Arthur Han

Kenneth J Dean

William R Carr

A new group of Os(II) and Os(III) compounds useful as redox mediators in electrochemical biosensors. These compounds have 1) low oxidation potential, 2) fast reaction kinetics between the electroactive center of an enzyme and the compound, 3) slow oxidation of osmium by oxygen, and 4) excellent solubility in aqueous medium. These mediators are particularly useful as a component of a reagent used in an electrochemical biosensor, wherein the biosensor is useful for measuring analytes from a biological fluid, such as blood.

Reagent including an osmium-containing redox mediator

The invention is a stable reagent used in assay systems that form a colored ferric ion complex, such as Prussian Blue. Such reagents are useful for the detection or measurement of an analyte from a fluid sample. Surprisingly, it has been found that the inclusion of certain ferric ion chelating agents, such as 3-sulfobenzoic acid, will inhibit formation of the blank reaction in the reagent.

Stable reagent for ferric ion complex indicator systems

Osmium-containing redox mediator

The invention describes a composition useful in determining sample analytes, where the determination is carried out anaerobically. The compositions include an analyte oxidizing agent, an electron transfer agent, ferric ions, and two chelators. The first chelator complexes to ferric ions, but does not have good affinity for ferrous ions. The second chelator does chelate ferrous ions, and forms a colored complex with the ion. It is the colored complex which serves as the indicator for the analyte. Different formulations of the composition are described.

Compositions useful in anaerobic determination of analytes

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Indianapolis, IN, United States of America

Walter W Jernigan