Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Walter Rittersdorf

Wolfgang Werner

Ulfert Deneke

Hans Wielinger

Werner Guthlein

Hartmut Merdes

Gerhard Hiller

Uwe Goebbert

Klaus Buecker

Peter Vogel

Peter Rieckmann

Wolfgang-Reinhold Knappe

Hans Lange

Dieter Berger

Anselm Rothe

Gunter Frey

Manfred Kuhr

Detlef Thym

Werner Guethlein

Manfred Bleisteiner

Hans-Georg Rey

Laszlo Koever

Gerd Zimmermann

Wolfgang Vomel

Rolf Nagel

Hugo Tiedemann

Heinz-Friedrich Trasch

Helmut Rehner

Adolf K Selle

Andreas Trost

Hans-Rudolf Lange

Hugo Tiedmann

Hanz-Georg Rey

Ernst-Werner Busch

Erwin Endres

An apparatus comprising a first porous carrier and a second porous carrier for evaluating biological fluid samples is disclosed. The apparatus is used for separating non high density lipoprotein (non-HDL) from a lipoprotein in a body sample and for determining high density lipoprotein (HDL) cholesterol in a HDL and non high density lipoprotein (non-HDL) in a body sample.

Method for the determination of HDL cholesterol by means of a rapid diagnostic agent with an integrated fractionating step

The subject matter of the application is a method for the separation of non-HDL lipoproteins from biological fluids in a carrier through which liquids can flow. The carrier contains a precipitating agent for non-high density lipoproteins (non-HDLs).

Method for separating non-HDLs from HDLs, and determining, HDL cholesterol

The invention relates to a method useful in separating non-high density lipoproteins, referred to as 'non-HDLs', from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDL-s are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.

Method for separating non-high density lipoproteins from lipoprotein

The invention relates to a method useful in separating non-high density lipoproteins, referred to as 'non-HDLs', from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.

Method for the determination of HDL cholesterol by means of a rapid

The invention relates to an apparatus and a method useful in separating non-high density lipoproteins, referred to as 'non-HDLs', from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.

Apparatus for determination of HDL cholesterol

The invention relates to a method for determining an ion in an aqueous sample. The method involves contacting the sample with a water immiscible material including an ionophore, a pH indicator and a compound which stabilizes sensitivity of the assay. The ion reacts with the ionophore in the water immiscible material, releasing a proton which reacts with the pH indicator generating a color change which is representative of the ion to be measured. The stabilizing compound stabilizes this change. Also described are compositions including the three recited elements, and various new compounds useful as the stabilizer.

Method for the determination of an ion with increased sensitivity, use

The subject matter of the present invention are naphthol derivatives having the general formula I ##STR1## in which R.sup.1, R.sup.2, R.sup.3 are the same or different and each represents hydrogen, an alkyl or alkoxy group, in which at least one of the residues is a (C.sub.8 -C.sub.30)-alkyl or alkoxy residue

Naphthol derivatives, processes for their production and their use

The present invention provides erythrocyte-retention substrates of the general formula:

Erythrocyte-retention substrates

The present invention provides a process for the detection of redox reactions by introducing a redox reagent system into a test system, wherein a soluble iodate is additionally added to the test system in an amount which is in excess of the highest amount of disturbing reducing agents present in the test system. The present invention also provides a diagnostic agent for the detection of redox reactions containing a redox reagent system, wherein the test system used additionally contains an iodate which is soluble therein in an amount which is in excess of the highest amount of disturbing reducing agents present in the test system.

Method for the determination of redox reactions using iodate to

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom and the method of preparation and use.

Agent for the determination of esterolytic and/or proteolytic enzymes

The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity. The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents:

Reagent and process for the determination of the activity of the enzyme

A cuvette for the determination of a chemical component of a fluid by photo evaluation has two planar-shaped parts parallel to one another at least one of which is transparent in the spectral range used for the evaluation. A planar distance piece of a filamentary reticulation for receiving the fluid therein spaces the planar-shaped parts apart to set the length of the light path through the cuvette for the photo evaluation.

Cuvette for the photo determination of chemical components in fluids

The present invention provides a process for removing ascorbic acid from aqueous solutions by the addition of ascorbate oxidase, wherein catalase and hydrogen peroxide are also added to the solution. The present invention also provides an agent for the removal of ascorbic acid from aqueous solutions, based on a solid carrier material impregnated with ascorbate oxidase, wherein the carrier material also contains catalase and hydrogen peroxide and optionally also a stabilizer and/or buffer substance.

Growing community of inventors

Mannheim, Germany

Walter Rittersdorf