Philip Morris Inc.

Philip Morris USA Inc.

Walter P Hempfling

Gordon H Bokelman

Marc Robert Krauss

Qinglin Li

Maria Shulleeta

Dick Hilliard

Newton E Kalengamaliro

Methods for increasing the concentration of antioxidants during senescence and curing of tobacco leaves include application of a chemical solution. The methods include spraying the solution onto the tobacco plant prior to harvest and/or applying the chemical solution to the soil surrounding roots of growing tobacco plants. The application of the chemical solution preferably occurs between topping and harvest, and is optimized to simulate the production of antioxidants and interfere with the formation of TSNAs during curing. The tobacco can be burley tobacco subjected to air curing.

Sodium chlorine spray treatment of the leaves present on tobacco plants to significantly reduce TSNAs in cured tobacco produced therefrom

Methods for increasing the concentration of antioxidants during senescence and curing of tobacco leaves include application of a chemical solution. The methods include spraying the solution onto the tobacco plant prior to harvest and/or applying the chemical solution to the soil surrounding roots of growing tobacco plants. The application of the chemical solution preferably occurs between topping and harvest, and is optimized to stimulate the production of antioxidants and interfere with the formation of TSNAs during curing. The tobacco can be burley tobacco subjected to air curing.

Sodium chloride spray treatment of soil surrounding tobacco plants to reduce TSNAs

Tobacco is treated before or during curing to lower or eliminate bacterial populations and/or activity, fungal growth, and/or tobacco-specific nitrosamine or bacterial endotoxin levels in the cured tobacco, wherein the tobacco is treated with an effective amount of a wash solution. Air-cured tobacco may be cured in four weeks or less when treated with a wash solution of bicarbonate salts.

Method for reduction of tobacco specific nitrosamines

Tobacco is treated with an effective amount of one or more bactericidal gases before or during curing to reduce or eliminate bacteria, bacterial activity and/or fungal activity from tobacco leaves, and/or to reduce or eliminate the amount of tobacco-specific nitrosamine or bacterial endotoxin in cured tobacco leaves. Cured tobacco is treated with an effective amount of one or more bactericidal gases before or during storage to reduce or eliminate bacteria, bacterial activity and/or fungal activity from the cured tobacco.

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Mechanicsville, VA, United States of America

Walter P Hempfling