Elitechgroup B.v.

Epoch Biosciences, Inc.

Elitech Holding B.v.

Ge Healthcare Dharmacon, Inc.

Dharmacon, Inc.

Walt Mahoney

Nicolaas M J Vermeulen

Irina Aleksandrovna Afonina

Anastasia Khvorova

Yevgeniy S Belousov

Jon Michael Karpilow

Annaleen Vermeulen

Rob Kaiser

Nicolaas Vermeulen

The present methods pertain to amplifying and/or detecting('SA') and methicillin-resistant('MRSA') nucleic acids based on a combined detection of ldh1 as a SA marker and mecA as a MRSA marker. In certain embodiments the methods also pertain to amplifying and/or detecting one or more SCCmec integration sites or bridge regions. Primers and probes are suitable to be used in the present methods to detect SA and MRSA simultaneously in a single reaction or in separate reactions. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (&#x201c;FRET&#x201d;), radiolabels, enzyme labels, and the like.

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Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders ('MGBs'). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.

Minor groove binder (MGB)-oligonucleotide miRNA antagonists

Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.

Amplification methods

Growing community of inventors

Woodinville, WA, United States of America

Walt Mahoney