Michigan State University

University of Copenhagen

Wajid Waheed Bhat

Björn Hamberger

Sean Johnson

Hans Jorgen Lyngs Jorgensen

Garret P Miller

Fani Ntana

Davis T Mathieu

David B Collinge

Emily R Lanier

Birgit Jensen

Constructs, host cells, fungi, seeds, plants, and methods are described herein can include aterpenoid synthase (SiTPS). Such constructs host cells, fungi, seeds, plants, and methods are useful, for example, for making viridiflorol. As described herein, the basidionycete, a non-specific-host root endophyte fungus, possesses a functional terpenoid synthase gene (SiTPS). Heterologous expression of SiTPS in host cells showed that the produced protein efficiently utilizes the fifteen-carbon precursor farnesylpyrophosphate (FTP) to synthesize the sesquiterpene alcohol viridiflorol, shown below.

Method for producing the sesquiterpene viridiflorol with a fungal enzyme

The disclosure relates to a method of making at least one serrulatane comprising contacting a terpene or a terpenoid substrate with at least one of a cis-prenyl transferase, a terpene synthase, and a cytochrome P45Q. The disclosure also relates to an expression system comprising one or more expression cassettes, each expression cassette comprising a promoter operably linked to a nucleic acid segment encoding at least one of: a cis-prenyl transferase, a terpene synthase, and a cytochrome P450. The disclosure also relates to a host cell comprising an expression system comprising one or more expression cassettes, each expression cassette comprising a promoter operably linked to a nucleic acid segment encoding at least one of: a cis-prenyl transferase, a terpene synthase, and a cytochrome P450.

Method to produce the anti-microbial diterpenoid leubethanol and related serrulatane-type diterpenes

Enzymes and methods are described herein for manufacturing terpenes, including terpenes.

Method for production of novel diterpene scaffolds

Growing community of inventors

Haslett, MI, United States of America

Wajid Waheed Bhat