Other

University of Utah Research Foundation

Idaho Technology, Inc.

Wade R Stevenson

Brian E Caplin

Jian Feng Chen

Carl Thomas Wittwer

Randy P Rasmussen

David J Eyre

Noriko Kusukawa

Deepika Marine deSilva

Andrew O Crockett

The present invention is directed to a nucleic acid quantification kit and method for determining the initial concentration or mass fraction of a target nucleic acid present in a sample. Illustrative embodiments include real-time competitive quantitative polymerase chain reaction (PCR) to determine the copy number or mass fraction of a target nucleic acid sequence in a sample and use of a thermodynamically based signal processing algorithm, with or without PCR, to provide mass fraction information.

Real-time gene quantification with internal standards

Probes and methods are provided for detection and analysis of nucleic acid sequences. The probes are single-labeled oligonucleotide probes whose fluorescence emission changes in response to probe-target hybridization and dissociation. The methods are for analyzing one or multiple nucleic acid loci using the probes. This invention further relates to the use of fluorescence changes in single-labeled probes for melting curve analysis, genotyping, and pathogen detection, and to methods for quantification of specific sequences in real-time monitoring of nucleic acid amplification.

Single-labeled oligonucleotide probes for homogeneous nucleic acid sequence analysis

Growing community of inventors

West Jordan, UT, United States of America

Wade R Stevenson