Dow Agrosciences LLC

Sangamo Therapeutics, Inc.

Corteva Agriscience LLC

W Michael Ainley

Steven Robert Webb

Lakshmi Sastry-Dent

Jeffrey C Miller

Dmitry Y Guschin

Zehui Cao

German Carlos Spangenberg

Jayakumar Pon Samuel

Matthew Hayden

Tim Sawbridge

Joseph F Petolino

Shreedharan Sriram

Debra L Camper

David Richard Corbin

Manju Gupta

Matthew J Henry

John White Forster

Noel Cogan

Lei Zhang

Ryan C Blue

Yidong Ran

Michael G Murray

John Mason

Liu Shen

Rebecca Ruth Miles

Cristie Min Dewes

Daniel Isenegger

Fyodor Urnov

James Wayne Bing

Ning Zhou

Gregory J Cost

Steven Lee Evans

Steven Arnold Thompson

Mary E Welter

Cristie M Dewes

Liu Y Shen

German Spangenberg

As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Optimal soybean loci

Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

Methods and compositions for integration of an exogenous sequence within the genome of plants

A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

Engineered landing pads for gene targeting in plants

A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Precision gene targeting to a particular locus in maize

Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

Methods and compositions for nuclease-mediated targeted integration of transgenes

The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

Rapid assay for identifying transformants having donor insertion

Growing community of inventors

Carmel, IN, United States of America

W Michael Ainley