University of California

Quest Diagnostics Investments, Inc.

Other

Lawrence Livermore National Security, LLC

Ion Dx, Inc.

Benner, W. Henry

Aguilar, Ben

Bogan, Michael, J.

W Henry Benner

Ronald M Krauss

Patricia J Blanche

Richard E Reitz

Michael P Caulfield

Earl Cornell

Gloria Kwangja Lee

Shuguang Li

John Dzenitis

Ben Aguilar

Michael J Bogan

William J Benett

Simon E Labov

Norman W Madden

Matthias Frank

Carl A Mears

Brian R Baker

Stephen Fuerstenau

William Searles

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

Lipoprotein analysis by differential charged-particle mobility

Apparatuses and methods are described for determining properties of ions travelling through a gas under the influence of an electric field. The apparatuses and methods can be understood to provide measurements of the electrical mobility of ions as useful for determining the electrical mobility constant Ko of electrosprayed substances, such as proteins. The apparatuses and methods relate to the scientific discipline of ion mobility spectrometry. Modules connected to ion mobility spectrometers provide stress to substances for the purpose of investigating, for example, the thermal stability of proteins. One form of the technology includes a tubular spectrometer body having an electrically conductive inner wall; a rod positioned along the longitudinal center of the body and electrodes positioned on, but electrically isolated from, the inner wall, where the ratio of the radius of the tubular spectrometer body to the ratio of the radius of the rod is at least 20.

Ion mobility spectrometer with center rod

Apparatuses and methods are described for determining properties of ions travelling through a gas under the influence of an electric field. The apparatuses and methods can be understood to provide measurements of the electrical mobility of ions as useful for determining the electrical mobility constant Ko of electrosprayed substances, such as proteins. The apparatuses and methods relate to the scientific discipline of ion mobility spectrometry. Modules connected to ion mobility spectrometers provide stress to substances for the purpose of investigating, for example, the thermal stability of proteins. One form of the technology includes a tubular spectrometer body having an electrically conductive inner wall; a rod positioned along the longitudinal center of the body and electrodes positioned on, but electrically isolated from, the inner wall, where the ratio of the radius of the tubular spectrometer body to the radius of the rod is at least 20.

Instruments are disclosed for analyzing ions from about 1000 to 10,000,000 Daltons by controlling a gaseous medium through which the ions travel under the influence of an electric field so that properties of the ions, such as diameter, electrical mobility, and charge, are measured. One embodiment of the disclosed instruments includes an ion source, a nozzle, a jet relaxation region, an ion accumulation region, an electronic gate, a flow chamber and an ion detector.

Instruments for measuring ion size distribution and concentration

Instruments are disclosed for analyzing ions from about 1000 to 10,000,000 Daltons by controlling a gaseous medium through which the ions travel under the influence of an electric field so that properties of the ions, such as diameter, electrical mobility, and charge, are measured. One embodiment of the disclosed instruments include an ion source, a nozzle, a jet relaxation region, an ion accumulation region, an electronic gate, a flow chamber and an ion detector.

Provided herein are fluidics platforms and related methods for performing integrated sample collection and solid-phase extraction of a target component of the sample all in one tube. The fluidics platform comprises a pump, particles for solid-phase extraction and a particle-holding means. The method comprises contacting the sample with one or more reagents in a pump, coupling a particle-holding means to the pump and expelling the waste out of the pump while the particle-holding means retains the particles inside the pump. The fluidics platform and methods herein described allow solid-phase extraction without pipetting and centrifugation.

Fluidics platform and method for sample preparation

Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing, elution, assay assembly, assay detection, and cleaning after reactions and between samples.

Fluidics platform and method for sample preparation and analysis

A medical diagnostic method and instrumentation system for analyzing noncovalently bonded agglomerated biological particles is described. The method and system comprises: a method of preparation for the biological particles; an electrospray generator; an alpha particle radiation source; a differential mobility analyzer; a particle counter; and data acquisition and analysis means. The medical device is useful for the assessment of human diseases, such as cardiac disease risk and hyperlipidemia, by rapid quantitative analysis of lipoprotein fraction densities. Initially, purification procedures are described to reduce an initial blood sample to an analytical input to the instrument. The measured sizes from the analytical sample are correlated with densities, resulting in a spectrum of lipoprotein densities. The lipoprotein density distribution can then be used to characterize cardiac and other lipid-related health risks.

Aerosol preparation of intact lipoproteins

Method of assessing a lipid-related health risk based on ion mobility analysis of lipoproteins

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Danville, CA, United States of America

W Henry Benner