Zymergen Inc.

Vytas SunSpiral

Sean Poust

Sara Da Luz Areosa Cleto

Kenneth S Bruno

Brian Chaikind

Arthur Muir Fong, Iii

Patrick J Westfall

Edyta Szewczyk

Hassan Abdulla

Dylan Vaughan

Jennifer Fredlund

Kyle Rothschild-Mancinelli

Paolo Boccazzi

Philip Weyman

William Serber

Matthew Jonathan Myers

A HTP genomic engineering platform for improving filamentous fungal cells that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols is provided. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. Methods for isolating clonal populations derived from individual fungal spores are also provided.

HTP genomic engineering platform for improving fungal strains

HTP genomic engineering platform for improving filamentous fungal cells that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. Methods can be carried out within optofluidic devices.

Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.

Liquid based selection and cell isolation

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Oakland, CA, United States of America

Vytas SunSpiral