Carl-zeiss-stiftung

Volker Wilke

Klaus Weber

Wilhelm Ansorge

Walter Geis

Gerhard Mueller

Albrecht Kettler

Werner Schmidt

Hubert Nasse

The invention is directed to a method for performing work on cells of a cell culture which includes microinjecting cells in the cell culture or drawing liquid from an individual cell or withdrawing whole cells with the aid of suction from the cell culture. The system is computer-supported and a marker movable in the image on a monitor is superposed on a television image of the cell culture. The marker is positioned in the television image on the cell to be injected by the operator by means of a relative movement of the marker with respect to the image. The coordinates of the cells marked in this way are stored by the computer which thereafter advances the capillary point automatically to the selected cells and penetrates the latter. Pursuant to a feature of an advantageous embodiment of the invention, the computer guides the penetration movement parallel to the longitudinal axis of the capillary by means of a superposed movement (k) of the object stage and through a superposed movement of the drive for the elevation displacement (z) of the capillary on the micromanipulator.

Method for performing work on cells of a cell culture and apparatus

The invention contemplates scanning-microscope display of plural observed parameters of an examined specimen, where the parameters derive from flying-spot light-exposure of the specimen, and where at least one of the observed parameters is outside the wavelength range of the flying-spot. In some illustrative embodiments, at least one of the observed parameters is sensed by a detector which uses the same scanning optics as the flying-spot, and in other embodiments other techniques of synchronization are involved. The disclosed embodiments also provide for selective arrest of scanning to enable such factors as fading fluorescence and spectrum analysis to be ascertained strictly for a surface occlusion, impurity or other anomaly of interest.

Method and apparatus for light-induced scanning-microscope display of

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Aalen, Germany

Volker Wilke