Bayer Aktiengesellschaft

Bayer Cropscience Ag

Bayer Healthcare LLC

Bayer Intellectual Property Gmbh

Volker Möhrle

Robert Velten

Rita Fröde

Günther Eberz

Peter Jeschke

Olga Malsam

Peter Lösel

Ulrich Ebbinghaus-Kintscher

Michael Edmund Beck

Rainhard Koch

Peter Schwan

Martin Lobedann

Walter Weichel

Rolandas Meskys

Benjamin Maiser

Markus Schindler

José A Salas

Yuval Shimoni

The invention provides a method for the continuous, microbe-reduced production and/or processing of a biopharmaceutical, biological macromolecular product from a heterogeneous cell culture-fluid mixture, comprising the steps of: (a) providing a particle-free fluid from a heterogeneous cell culture-fluid mixture containing the product, in the form of a product stream, (b) at least one filtration, providing a filtrate, (c) at least two chromatography steps for purifying the product, (d) at least one virus depletion, (e) at least one ultrafiltration and/or at least one diafiltration of the product stream of steps (b), (c) and/or (d), characterized in that the at least two chromatography steps from (c) comprise a purification via at least two chromatography columns and/or membrane adsorbers in each case and that the process is carried out in a closed and modular manner The invention further provides a corresponding modular system for carrying out said method.

Modular system and process for the continuous, microbe-reduced production and/or processing of a product

The present invention provides a compounded cell culture medium powder formulation comprising: a basal medium powder and a cell culture media supplement, wherein the cell culture media supplement comprises and one or more salts; one or more growth factors; one or more inorganic ions; an amino acid supplement comprising one or more of asparagine, glutamine, histidine, and serine; one or more buffers; and one or more anti-foaming agents. The invention further provides methods of making a compounded cell culture medium powder formulation methods of making a cell culture medium for growing mammalian cells and methods of producing a protein of interest by culturing cells in the cell culture medium and isolating the protein of interest.

Compounded media powder formulation and method of preparation of liquid medium for cell culture

The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) which have an increased substrate specificity compared with the wild type, and also to methods for production and identification thereof.

Variants of PQQ-dependent glucose dehydrogenase having improved substrate specificity

The present invention relates to derivatives of 9-keto spinosyns, which are substituted by a &#x2550;N&#x2014;(O, NH or NR)&#x2014;Rmoiety in the C-9 position, to methods for their manufacture, and to their use for controlling animal pests.

9-ketospinosyn derivatives

The present invention relates to nucleic acid coding for enzyme activities of spinosyn biosynthesis and to the relevant enzymes per se. Furthermore, the invention relates to methods for preparing spinsoyn derivatives and spinosyn precursors.

Nucleic acids which code for the enzyme activities of the spinosyn biosynthesis

The present invention relates to methods for producing novel spinosyn derivatives which are substituted with a 1-hydroxy-ethyl radical in the C-21 position and to novel spinosyn derivatives of this type per se and to their use for producing novel spinosyns.

Method for producing novel spinosyn derivatives

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Kö'ln, Germany

Volker Möhrle