Miltenyi Biotec, Gmbh

Miltenyi Biotec B.v. Co., Kg

Volker Huppert

Stefan Miltenyi

Andrew Kaiser

Burgund Kauling

Christian Dose

Wing Leung

Ian Johnston

Rafijul Bari

Markus Granzin

Nina Moker

Philipp Steinbrück

Michael Essl

Rupert Handgretinger

Alexander Scheffold

Christian Seitz

Peter Lang

Patrick Schlegel

Julia Dzionek

Katharina Oerding

Nina MÖKER

M Burgund Kauling

Mike Essl

Sabine Müller

The present invention discloses an in-vitro method for transferring biological material into activated NK cells with a pseudotyped retroviral vector particle or a virus-like particle thereof, comprising the steps a) activation of NK cells, and b) addition of said pseudotyped retroviral vector particle or virus-like particle thereof to said activated NK cells, wherein said pseudotyped retroviral vector particle or virus-like particle thereof comprises a modified baboon endogenous retrovirus (BaEV) envelope glycoprotein that is able of binding to and fusing with a hematopoietic cell membrane, thereby transferring biological material into said activated NK cells. Preferentially, the activating of NK cells is performed by the addition of a IL-1 family cytokine to the NK cells.

Method for NK cell transduction

The present invention provides methods and compositions for separating cells from a sample containing erythrocytes. The method is for recovering desired cells from a sample containing the desired cells, erythrocytes and undesired cells comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocytes aggregation reagent ii) at least one antigen recognizing moiety coupled to a magnetic particle, wherein said particle with said at least one antigen recognizing moiety specifically binds to at least one antigen specific for one or more undesired cellular components; b) applying simultaneously i) gravity sedimentation for sedimentation of erythrocytes and ii) a magnetic field gradient to said sample for immobilizing said magnetic particle generating a pellet and a supernatant phase, and c) recovering the desired cells from the supernatant phase. Compositions for the use within the present method are also disclosed.

Technology for purifying NK cells and other cell types by concurrent gravity sedimentation and magnetic separation

The invention is directed to a method for enriching target cells from a sample of cells characterized by:

Multisort cell separation method

The invention relates to a process for generation of genetically modified stem cells comprising the steps

Automated generation of genetically modified stem cells

Growing community of inventors

Kürten, Germany

Volker Huppert