Evonik Degussa Gmbh

Forschungszentrum Julich Gmbh

University of California

Eth Zurich

Rijksuniversiteit Groningen

Sintef Tto as

Universitat Bielefeld

Volker F Wendisch

Brigitte Bathe

Georg Thierbach

Bernhard Eikmanns

Bastian Blombach

Verena Engels

Hermann Sahm

Caroline Kreutzer

Wilfried Claes

Trygve Brautaset

Sydney G Kustu

Jean-Charles Portais

Stéphanie Heux

Nadja Alina Henke

Doris Rittmann

Steffen Lindner

Eva Potthoff

Petra Peters-Wendisch

Tonje Marita Bjerkan Heggeset

Anne Krog

Wilhelmus Johannes Quax

Mark Jan Jacobus Bernhard Sibbald

Julia Vorholt

Jonas Müller

Patrick Kiefer

Lennart Lessmeier

Caroline Gerth

Tonje Marita Heggeset

Jonas Muller

Stephanie Heux

Mark Jan Sibbald

The present invention provides a method of producing astaxanthin and lysine in recombinant gram-positive bacteria comprising a nucleic acid sequence encoding for a crtZ-protein fromand comprises a nucleic acid sequence encoding for a crtW-protein.

Carotenoid and amino acid biosynthesis using recombinant corynebacterium glutamicum

The present invention relates to a nucleic acid molecule, which encodes a polypeptide having alcohol dehydrogenase activity, in particular methanol dehydrogenase activity, comprising having a nucleotide sequence selected from the group consisting of: (i) a nucleotide sequence as set forth in any one of SEQ ID NOs: 1 (mdh2-MGA3), 3 (mdh3-MGA3), or 5 (mdh2-PB1); (ii) a nucleotide sequence having at least 90% sequence identity, more particularly at least 91, 92, 93, 94, 95, 96, 97, 98 or 99% sequence identity, with a nucleotide sequence as set forth in any one of SEQ ID NOs: 1, 3 or 5; (iii) a nucleotide sequence which is degenerate with any one of the nucleotide sequences of SEQ ID NOs: 1, 3 or 5; (iv) a nucleotide sequence which is a part of the nucleotide sequence of any one of SEQ ID NOs: 1, 3 or 5, or of a nucleotide sequence which is degenerate with a sequence of SEQ ID NOs: 1, 3 or 5; (v) a nucleotide sequence encoding all or part of a polypeptide whose amino acid sequence is set forth in any one of SEQ ID NOs: 2 (Mdh2-MGA3), 4 (Mdh3-MGA3) or 6 (Mdh2-PB1); and (vi) a nucleotide sequence encoding all or part of a polypeptide which has an amino acid sequence having at least 90% sequence identity, preferably at least 91, 92, 93, 94, 95, 96, 97, 98 or 99% sequence identity, with an amino acid sequence as set forth in any one of SEQ ID NOs: 2, 4 or 6; or a nucleic acid molecule comprising a nucleotide sequence which is complementary to the nucleotide sequence of any one of (i) to (vi). Also provided are recombinant constructs, vectors and host cells comprising such a nucleic acid molecule and polypeptides encoded thereby. Such molecules may advantageously be used in the genetic modification of host cells, for example to introduce or modify methanol dehydrogenase activity.

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The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NADkinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NADkinase is overexpressed.

Method for fermentatively preparing L-amino acids

A method for producing an L-amino acid comprising: a) cultivating in a medium containing glycerol a recombinant coryneform bacteria which produces the desired L-amino acid and which expresses at least one heterologous polynucleotide of glycerol metabolism, such as glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, gipT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and/or talC, and b) isolating the desired L-amino acid. Preferably, the pathways producing the desired L-amino acid in the coryneform bacteria are amplified.

Method for the fermentative production of L-amino acids with the aid of coryneform bacteria capable of using glycerin as the only carbon source

The invention relates to a recombinant coryneform bacterium which secretes an organic chemical compound and in which the sugR gene which codes for a polypeptide having the activity of an SugR regulator has been attenuated. The invention further relates to a processes for using this bacterium for the fermentative preparation of organic chemical compounds.

Process for the fermentative preparation of organic chemical compounds using Coryneform bacteria in which the sugR gene is present in attenuated form

Process for the fermentative preparation of organic chemical compounds using coryneform bacteria in which the SugR gene is present in attenuated form

The invention provides methods and compositions for selectively isolating total bacterial mRNA. The general method comprises contacting a bacterial lysate comprising total bacterial mRNA and nonisolated bacterial polysomes with an exogenous enzyme under conditions wherein the enzyme selectively modifies the mRNA to form modified mRNA, and isolating the modified mRNA. In particular embodiments, the enzyme is selected from a poly(A) polymerase, a RNA ligase and a terminal deoxynucleotidyl transferase. Depending on the enzyme, the modified mRNA may have any of a variety of modifications, such as a 3&prime; tail, particularly a poly(A) tail, a specific sequence tag, a detectably labeled nucleotide, etc.

Selective isolation of bacterial mRNA

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Juelich, Germany

Volker F Wendisch