Carl Zeiss Microscopy Gmbh

Volker Doering

Alexander Freytag

Wibke Hellmich

Andreas Seher

Rebecca Elsaesser

A reference sample for calibrating and/or adjusting a microscope and to uses of the reference sample. The latter has at least one carrier structure made of a carrier material which, at least in regions of its extent, is excitable to emit luminescence light, and at least one two-dimensional and/or three-dimensional structure consisting of a number of substructures. The carrier material is diamond or silicon carbide and is doped in or around the regions of the two-dimensional and/or three-dimensional structure in order to be excitable to emit the luminescence light.

Reference sample for a microscope, methods and uses

A prediction algorithm determines synthetic fluorescence images on the basis of measurement images. A validation of the synthetic fluorescence images can be effected on the basis of reference images which are captured after the measurement images or are captured for a separate sample. Alternatively or additionally, a training of the prediction algorithm can be effected on the basis of training images which are captured after the measurement images or are captured for a separate sample.

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Jena, Germany

Volker Doering