Monsanto Technology LLC

Calgene Corporation

Vladimir Sidorov

Guangning Ye

Yuechun Wan

Jeffrey M Staub

David M Stalker

David R Duncan

Charles L Armstrong

Narender S Nehra

David J Schaaf

A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

Plastid transformation of maize

A method is provided for transforming maize plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of maize plant tissue with heterologous DNA constructs by microprojectile bombardment of green light grown cultures and dark grown embryogenic cultures. The invention also provides for maize cells in which the plastids contain heterologous DNA constructs.

The present invention relates to a novel system for generating transformed dihaploid plants from haploid cells and tissues without the use of chromosome doubling agents.

Method for agrobacterium transformation for dihaploid corn plants

A method is provided for transforming solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5&prime; to 3&prime; direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilized in the transformation of solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid.

Growing community of inventors

Chesterfield, MO, United States of America

Vladimir Sidorov