Life Technologies Corporation

Molecular Probes, Inc.

State of Oregon

Vladimir Martin

Kyle R Gee

Aleksey Rukavishnikov

Daniel Beacham

Jeffrey Dzubay

Richard P Haugland

Brian Agnew

Jolene Ann Bradford

Zhenjun Diwu

John F Keana

Wayne Forrest Patton

Joseph M Beechem

Jixiang Liu

William H Ralston

Thomas H Steinberg

A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I:

Fluorogenic pH sensitive dyes and their method of use

A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.

A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: 

The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.

Fluorescent metal ion indicators with large stokes shifts

Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (M), a reporter molecule and two or more lipophilic groups (G) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups. An exemplary compound for use as a cell permeable metal ion indicator is represented by the structure: 

Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol

Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators.

Benzoxazole-based fluorescent metal ion indicators

The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a &#x2014;N,N,O-triacetic acid analog of BAPTA and has the following formula 

Heavy metal binding compounds and their method of use

The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety has the following formula 

Fluorescent ion indicators for cadmium and lanthanide ion detection

Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (M), a reporter molecule and two or more lipophilic groups (G) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.

The invention describes crown ether chelators, including crown ethers having the formula: 

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Eugene, OR, United States of America

Vladimir Martin