Celltronix

Vladimir Fedorovich Bolyukh

Igor Katkov

Pamela Itkin-Ansari

Valery Sergeevich Lupikov

The present invention, in some embodiments thereof, relates to a method and scalable devices for hyperfast cooling and re-warming of samples. More specifically, it relates to cryogenic preservation of biological samples via vitrification. It includes: a liquid sample placed at ambient temperature in a flat thermo conductive container that in some embodiments additionally contains a detachable disposable or sterilizable thermo conductive spiral; transferring the sample to a cooling chamber using a linear percussion stepping motor drive; hyperfast cooling of the sample using streams of pressurized liquid coolant; transferring the sample to a detachable shipping/storage chamber filled with liquid coolant, from which the sample can be transferred to another vessel that contains liquid cryogenic coolant and moved back to the shipping/storage chamber. This chamber can be then attached to a re-warming chamber, in which the sample is heated to a biologically tolerant temperature above 0 degrees Celsius in a hyperfast manner.

Method and scalable devices for hyper-fast cooling and warming

A method for hyper-fast cooling of samples is described. The samples are secured in a sample container, then the sample container inserted into a cryogenic cooling chamber through an opening of the cooling chamber. The sample container is placed some distance away from a cooling head, but within range of a continuous stream of pressurized liquid coolant emitted by the cooling head, where, the sample is hyper-cooled by spraying the sample container with a continuous stream of the pressurized liquid coolant.

Method and scalable devices for hyper-fast cooling

Embodiments of a cryopreservation device and method of use are described that allow cryopreserved samples to be transferred from one location to another. In one embodiment, a cryopreservation device comprises two, telescoping tubes; one for plunging biological material into a reservoir of cryogenic liquid refrigerant, and the other for capturing the biological material along with some of the liquid refrigerant, for transferring the biological material to another location.

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Kharkiv, Ukraine

Vladimir Fedorovich Bolyukh