University System of Maryland

Nlt Technologies, Ltd.

Vjekoslav Pavlin

Douglas A Newberg

Richard R Newberg

Jackson C Yang

An apparatus for inoculating a sample to or withdrawing a sample from a vessel or conduit includes a body with an internal sample cavity, a valve operating rod movable to open and close an orifice to the sample cavity a coupler to attach the body to a port of the vessel or conduit. A manual and/or automatic device can be provided for moving the valve operating rod to open and close the orifice. The manual device includes a trigger actuator. A portion of the sample cavity is formed by an endcap which includes the orifice. The sample cavity has an inner wall and outer wall with a space therebetween. The space can thermally insulate the sample cavity from the ambient environment as well as the interior of the vessel or conduit. The valve operating rod has a diaphragm attached to one end. A blunt sealing tip of the diaphragm seals the orifice when the valve operating rod moves to close the orifice. The valve operating rod has a portion which extends into the diaphragm to support the diaphragm and to form a seal at an opening to the sample cavity through which the diaphragm extends.

Automated sample extractor or feeder/inoculator for bioreactors and

An apparatus can withdraw a sample from a vessel or conduit or provide a sample to this vessel or conduit. This apparatus includes a main body with an inlet passage and drain passage. The main body has a longitudinal axis with the axes of the drain passage and inlet passage being inclined relative thereto. A flexible bellows is positioned within an internal cavity of the main body. A sealing tip of the bellows is movable by a valve operating rod to close an orifice to a sample cavity within the body. A sample can be taken from the vessel or conduit, through this sample cavity and out the drain passage to a sampling device when the apparatus is used as a sampler. On the other hand, a sample can be fed through the inlet passage, sample cavity and orifice to the vessel or conduit when the apparatus is used as a feeder/inoculator. Steam, pure dry air and/or a wash medium can be provided through the inlet passage to cleanse the apparatus. Crevices and exposure to moving parts are avoided in the apparatus to prevent contamination of the sample. This apparatus can be retrofitted to existing containers or vessels and can be automatically controlled and monitored.

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Adelphi, MD, United States of America

Vjekoslav Pavlin