Danisco US Inc.

Oracle International Corporation

Movable, Inc.

The Goodyear & Tire Rubber Company

Movable Ink

Vivek Sharma

Jayarama K Shetty

Gopal K Chotani

Michael Jay Pepsin

Sung Ho Lee

Paula Johanna Maria Teunissen

Suzanne Breneman

William Albert Cuevas

Gang Duan

Glenn E Nedwin

Thomas S Green

Bruce A Strohm

Bradley A Paulson

Ying Qian

Hongxian Xu

Michael Nutt

Michael Bowker

Keith Majkut

Srinivasu Gottipati

Bradley Paulson

Andrew Shaw

Scott Douglas Power

James T Kellis, Jr

Luis Gustavo Cascao-Pereira

Sandra W Ramer

Sang-Kyu Lee

Anthony R Calabria

Donald E Ward

Jaclyn Diana Demartini

Jacob Andrew Latone

Sundeep Nayak

David E Wildes

Floor K Kooy

Craig B Yappert

Luis G Cascāo-Pereira

Collette M Blake

Douglas Ko

Matt Reboli

Pauline Johanna Maria Teunissen

Dina Finan

Martien H Bergsma

William A Cuevas

James T Kellis

The present invention relates to a dry grind ethanol process in which post-distillation backset is subjected to aerobic fermentation to remove components that are inhibitory to an ethanolagen, such as yeast, allowing the utilization of an increased amount of post-distillation backset during the initial preparation of starch-containing substrates for the dry grind ethanol process. Aerobic fermentation of the post-distillation backset allows substantially higher backset recycle, resulting in fresh water savings and increased sustainability.

Method for increasing backset recycle in dry grind alcohol production

Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

Alpha-amylase combinatorial variants

Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence ofglucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.

Methods for saccharifying a starch subtrate

Systems and methods to manage dynamic content in email via an image server. The image server being configured to communicate and receive requests from an email client regarding dynamic content. The image server is also configured to load data regarding the email client from an asset database, and to communicate with a decision engine. Further, the image server is configured to communicate with a content acquisition server, to communicate an analytics database, and to cause display of dynamic content in an email.

Management of dynamic email content

Methods for saccharifying a starch substrate

Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism, such as isoprene, isoprenoid precursor molecules, and/or isoprenoids. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids

The present disclosure relates to aalpha-amylase (AmyE) or its variants thereof. AmyE or its variants thereof may be used to more efficiently produce fermentable sugars from starch. Also disclosed are a composition comprising a glucoamylase and AmyE or variant thereof and a method of processing starch utilizing the described enzyme composition.

Saccharification enzyme composition and method of saccharification thereof

Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

Production of isoprenoids under neutral pH conditions

Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment.

Compositions and methods for grain processing without pH adjustment

The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and aalpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of thealpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and thealpha-amylase, simultaneously or sequentially.

Alpha-amylase blend for starch processing and method of use thereof

The present invention relates to an alpha-amylase blend, including aalpha-amylase (AmyS) wherein the amino acid at position S242 is substituted and aalpha-amylase The invention also relates to processes using the alpha-amylase blends for starch liquefaction and saccharification, ethanol production, and a sweetener production.

Alpha-amylase blends and methods for using said blends

Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

Alpha-amylase variants with altered properties

Growing community of inventors

North Liberty, IA, United States of America

Vivek Sharma