Eni-Ente Nazionale Idrocarburi

Snamprogetti

Anic S.p.a.

Vittorio Baroncelli

Ivo Giannini

Filippo Porcelli

Claudio Colapicchioni

Pierluigi Renzi

New immunofluorescence reagents prepared by reacting together a protein, a cross-linking agent and a dye, which are of general formula ##STR1## and which fluoresce between 600 and 900 nm.

Immunofluorescence reagents, and the method for their preparation

The invention relates to a composition suitable for testing biological tissues and/or liquids, the composition consisting of:

Composition suitable for testing biological tissues and/or liquids, and

A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is intended for using the above specified composition in diagnostic runs. The method, briefly resumed, consists removing the tri-iodo thyronine of the serum sample being assayed by means of an insolubilized anti-T.sub.3 antibody and treating the residue with the sensibilized enzyme which is nothing else than the covalent formed by tri-iodo thyronine and an appropriate enzyme, such as carbonic anhydrase.

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Rome, Italy

Vittorio Baroncelli