Leland Stanford Junior University

Magarray, Inc.

The Scripps Research Institute

Viswam Siva Nair

Sanjiv Sam Gambhir

Shan X Wang

Utkan Demirci

Anders Carlsson

Heng Yu

Peter Kuhn

Fei Liu

Michael J Beggs

Luis Carbonell

Seung-min Park

Dawson Wong

Chin Chun Ooi

Anand Kolatkar

Shan Xiang Wang

A device ('the ExoTIC device') for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.

Exosome-total-isolation-chip (ExoTIC) device for isolation of exosome-based biomarkers

Exosome-Total-Isolation-Chip (ExoTIC) device for isolation of exosome-based biomarkers

Aspects of the present disclosure include methods of producing a circulating analyte profile of a subject. The methods include contacting a blood sample from a subject with a panel of probes for specific binding to analytes, and detecting the presence or absence of binding of the analytes to probes of the panel of probes. Also provided are sensor devices including a panel of capture probes and useful, e.g., for practicing the methods of the present disclosure.

Protein and autoantibody biomarkers for the diagnosis and treatment of lung cancer

A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.

Molecular analysis using a magnetic sifter and nanowell system

Growing community of inventors

Menlo Park, CA, United States of America

Viswam Siva Nair