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Ustav Organicke Chemie a Biochemie Av Cr, V.v.i.

Vysoka Skola Chemicko-technologicka V Praze

Joshi, Vishwas

Vishwas Joshi

Miroslav Hajek

Filip Teply

Manoj R Sonawane

Veronika Komarkova

Jana Novotna

Michael Jirasek

Jaroslav Kozak

Lukas Severa

Pavla Hubalkova

U M Sreenivasa Murthy

Shailendra Devicharan Rane

Erika Kuzmova

Paul Eduardo Reyes-Gutierrez

Manasi Sanjay Nade

This invention comprises: compositions comprising a derivative, plasmids, a reagent kit and methods of making these compositions a derivative, vaccine- and non-vaccine-compositions of above for causing death of cancer cells that form part of a tunoour and virus infected Denguue, Measles and other diseased cells; the derivative comprising replicating as well as non-replicating dervivaties of an attenuated negative stranded RNA virus belonging to family paramyxoviridae, including Measles Virus, comprising a single additional transcriptional unit carrying either only one or two or more non-viral genes, and the non-replicating derivatives being free from contaminating replicating Measles Virus (b) a Measles Virus packaging cell line for making above compositions, expressing the M, F and H proteins of MV stably. And (c) a reagent kit for producing the Measles Virus derivatives describved above.

DNA molecules producing custom designed replicating and non-replicating negative stranded RNA viruses and uses there of

Described is a simple plasma-based mammalian expression system that uses the RNA dependent RNA polymerase (RDRP) enzyme systems' activity for expression of recombinant proteins or RNA from viral minigenomes and rescue of recombinant viruses from cDNAs encoding entire genome(s) of negative stranded RNA viruses for use in synthesizing recombinant viruses and developing vaccines. This system will be used for expression of recombinant proteins, therapeutic RNA molecules including anti-sense and/or selecting interfering RNA and Ribozymes. This system can also be used for gene therapy and producing recombinant viruses for production of new vaccines.

Two plasmid mammalian expression system

Helquat derivatives of general formula I, in which substituents Rand Rare independently selected from a group comprising H and Cto Calkyl, up to three of S, S, Sand Sare present, each of S, S, Sand Sindependently represents a linker consisting of a bivalent hydrocarbon chain having 3-6 carbon atoms, preferably hydrocarbon chain having 4 carbon atoms, and one to four atoms selected from the carbon atoms with the descriptor 2, 4, 2', and 4&#x2032; are substituted with a substituent Rof general formula II, wherein Ris substituted or unsubstituted heteroaryl, Tand Tare independent linkers that bridge atoms Nwith Cand Nwith C, wherein Tand Tindependently represent a bivalent hydrocarbon chain having 2-5 carbon atoms, preferably 2 or 3 carbon atoms; and anions (X)and (X)independently represent anions of pharmaceutically acceptable salts.

Helquats with heteroaromatic substituents, preparation thereof, and use thereof as G-quadruplex stabilizers

Reverse engineering has offered new ways of studying the pathology of RNA viral infections, new more efficient devices of synthesizing recombinant viruses and developing vaccines and also demonstrated the versatility and efficiency of RNA dependent RNA polymerase RDRP system as an expression system. However, the currently used methods require a repertoire of complex, difficult-to-use tools. Present invention describes, a simpler plasmid based mammalian expression system that uses the RDRP enzyme activity for expression of recombinant proteins or RNA from viral minigenomes and rescue of recombinant viruses from cDNAs encoding entire genome(s) of negative stranded RNA viruses. This system will be useful for expression of recombinant proteins, therapeutic RNA molecules including anti-sense and/or selecting interfering RNA and Ribozymes. This system can also be used for gene therapy and producing recombinant viruses for production of new vaccines.

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Maharashtra, India

Vishwas Joshi