University of Utah Research Foundation

Biofire Defense, LLC

Idaho Technology, Inc.

Biofire Diagnostics, LLC

Virginie E Dujols

Carl Thomas Wittwer

Luming Zhou

Gudrun Reed

Philip Seth Bernard

Joseph A Holden

Carlynn Willmore-Payne

C Wade Dummer

Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Nucleic acid melting analysis with saturation dyes

Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Amplicon melting analysis with saturation dyes

AMPLICON melting analysis with saturation dyes

Methods of identifying the type of a cell are provided, the methods comprising determining the Tprofile of a sample rRNA or fragment thereof from the cell using a double stranded nucleic acid-specific dye, wherein a match between the determined Tprofile and the Tprofile of a corresponding rRNA or fragment thereof of a cell from a known cell type indicates that the sample rRNA is from the known cell type.

Species identification by melting analysis of secondary structure of single stranded nucleic acids

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Virginie E Dujols