Arbor Vita Corporation

Becton Dickinson and Company Limited

Virginia M Crews

John D Mantlo

Peter S Lu

Adrien P Malick

Johannes Schweizer

Stephen J Lovell

Julie L Rosales

Carrie S Ferguson

Nancy Hasse

Jeff H Bruton

Robert J Beadenkopf

Lydia Blank

Jefferey H Bruton

Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

Method of efficient extraction of protein from cells

Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.

Method of protein extraction from cells

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Virginia M Crews