The Radiochemical Centre Limited

Virginia Edith Chambers

Reginald Monks

Russell James Bayly

Anthony Leonard Riley

Folic acid and related compounds are assayed by competitive radio-assay using a selenium - 75 labelled version of folic acid as the competing compound.

Saturation analysis of folate compound with selenium-75 labeled folate

The invention provides a method and a kit for the saturation analysis of a steroid characterized by the use of a selenium-75 labelled version of the steroid to be assayed. Selenium-75 derivatives of steroids are quite simple to prepare and have practical advantages over other radioactively labelled steroids. Lists of derivatives and examples of competitive radio-assays are given.

Saturation analysis

Novel dimeric and monomeric selenium-75 derivatives of steroids are made by reacting a keto steroid with selenium-75 dioxide or selenious acid-Se75. The resulting diselenide dimers can be converted into monomeric compounds by the use of a cleaving reagent followed by an alkylating agent. The compounds are useful in saturation analyses such as radioimmunoassays.

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Amersham, United Kingdom

Virginia Edith Chambers